Determination of the optimal concentration and duration of C5aR antagonist application in an inflammatory model of human dental pulp cells. Issue 3 (20th February 2023)

• Record Type: Journal Article
• Authors: Hu, Junlong; Tan, Xiaohan; Wei, Xiaoling; Hu, Weiping; Gao, Li; Cao, Xiaofang; Yang, Huiying; Jiang, Zhuling; Li, Ning; Teng, Li; Liu, Mingyue
• Is Part Of: FEBS open bio. Volume 13:Issue 3(2023)
• Page Start: 570
• Page End: 581
• Publication Date: 2023-02-20
• Subjects: C5aR -- C5aR antagonist W54011 -- dental pulp cells -- interleukin‐6 -- lipopolysaccharides -- tooth decay; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1002/2211-5463.13571
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : Deep tooth decay approaching the pulp may develop into pulpitis; to prevent this, pulp cells need to balance the rapid immune response to avoid rapid swelling of the pulp. Current treatment of deep decay that approaches the pulp involves the application of drugs that induce low‐level inflammation in the dental pulp to promote its repair, but this treatment is sometimes insufficient. However, the unsuccessful treatment often resulted in pulpitis. The C5a‐C5aR is the initial stage of the immune cascade response. Blocking the binding of C5a‐C5aR can slow the immune response in the narrow pulp cavity, so that dental pulp cells have enough time to proliferate, migrate, and differentiate. In this study, we compared lipoteichoic acid (LTA) and lipopolysaccharides (LPS) at different concentrations and time points and used the C5aR antagonist W54011 to block the C5a‐C5aR axis. The blocking effect was detected by analyzing the expression of C5a, C5aR, interleukin (IL)‐6, and Toll‐like receptors 2 and 4 ( TLR‐2, 4) . Next, we determined the optimal concentration and duration of LTA and LPS treatment in combination with W54011. Based on our results, we selected 1.0 μg·mL −1 LPS treatment for 48 h to generate an inflammatory model of human dental pulp cells. We then regrouped the cells and conducted expression analyses to monitor the expression of C5a, C5aR, IL‐6, and TLR‐4 at the protein and mRNA levels. LPS stimulation for 48 h and treatment with W54011 for 48 h effectively inhibited inflammation and did not affect C5a expression. This study provides a basis for follow‐up studies of W54011 in dental pulp cells. Abstract : The key mechanism of successful pulp preservation is the balance between immunity and regeneration. C5a binds to its receptor and plays the strongest role in dental pulp immunity. To block the interaction between C5a and C5aR by using C5aR antagonists can downregulate the expression of IL‐6, which helps to slow down the immune process, which in turn can facilitate pulp repair.