Functional Skeletal Muscle Regeneration Using Muscle Mimetic Tissue Fabricated by Microvalve‐Assisted Coaxial 3D Bioprinting. Issue 7 (12th December 2022)

• Record Type: Journal Article
• Authors: Lee, Hanna; Kim, Soon Hee; Lee, Ji Seung; Lee, Young Jin; Lee, Ok Joo; Ajiteru, Olatunji; Sultan, Md Tipu; Lee, Suk Woo; Park, Chan Hum
• Is Part Of: Advanced healthcare materials. Volume 12:Issue 7(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-12-12
• Subjects: 3D bioprinting -- coaxial nozzles -- hydrogels -- microvalves -- skeletal muscles; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.202202664
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract: 3D‐printed artificial skeletal muscle, which mimics the structural and functional characteristics of native skeletal muscle, is a promising treatment method for muscle reconstruction. Although various fabrication techniques for skeletal muscle using 3D bio‐printers are studied, it is still challenging to build a functional muscle structure. A strategy using microvalve‐assisted coaxial 3D bioprinting in consideration of functional skeletal muscle fabrication is reported. The unit (artificial muscle fascicle: AMF) of muscle mimetic tissue is composed of a core filled with medium‐based C2C12 myoblast aggregates as a role of muscle fibers and a photo cross‐linkable hydrogel‐based shell as a role of connective tissue in muscles that enhances printability and cell adhesion and proliferation. Especially, a microvalve system is applied for the core part with even cell distribution and strong cell–cell interaction. This system enhances myotube formation and consequently shows spontaneous contraction. A multi‐printed AMF (artificial muscle tissue: AMT) as a piece of muscle is implanted into the anterior tibia (TA) muscle defect site of immunocompromised rats. As a result, the TA‐implanted AMT responds to electrical stimulation and represents histologically regenerated muscle tissue. This microvalve‐assisted coaxial 3D bioprinting shows a significant step forward to mimicking native skeletal muscle tissue. Abstract : This microvalve‐assisted co‐axial 3D bioprinting technology supports myotube maturation and functional regeneration in vitro and in vivo. The microvalve system promotes cell‐to‐cell interactions without a bioink, forming matured myotubes in the core, and finally helping to form a functional muscle fascicle. This fabrication strategy demonstrates the potential for fabricating functional skeletal muscle tissue capable of restoring injured skeletal muscle.