Alpinate Oxyphyllae extracts enhance the longevity and homing of mesenchymal stem cells and augment their protection against senescence in H9c2 cells. Issue 7 (4th December 2018)

• Record Type: Journal Article
• Authors: Chang, Yung‐Ming; Asokan Shibu, Marthandam; Tsai, Chuan‐Te; Tsai, Chin‐Chuan; Lin, Shu‐Luan; Chang, Chia‐Cheng; Viswanadha, Vijaya Padma; Chen, Ray‐Jade; Chang, Julia Huei‐Mei; Huang, Chih‐Yang
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 7(2019:Jul.)
• Page Start: 12042
• Page End: 12050
• Publication Date: 2018-12-04
• Subjects: aging -- CXCR -- mesenchymal stem cells -- SDF1 -- stem cell preconditioning; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.27867
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Adipose‐derived mesenchymal stem cells (ADMSCs) are easily accessible and are attractive mesenchymal stem cells for use in regenerative medicine; however their application is frequently restricted due to various challenges present in the environment they are administered. Therefore ADMSCs are preferably preconditioned with various stimulating factors to overcome the barriers developed in any pathological conditions. Here we used ADMSCs from rat adipose based on the abundance of positive markers and preconditioned the cells with extracts from Alpinate Oxyphyllae Fructus (AOF), a traditional Chinese herb used for antiaging, associated various health benefits. The preconditioned stem cells were tested for their potential to drive H9c2 from doxorubicin (Dox)‐induced aging. The AOF‐treated stem cells enriched stemness in ADMSCs with respect to their stem cells' positive marker, and enhanced their longevity mechanism and elevated the stem cell homing‐associated C‐X‐C chemokine receptor type 7 (CXCR7). The AOF preconditioned stem cells, when cocultured with H9c2 cells, showed effective protection to Dox‐induced senescence and stem cell homing to damaged H9c2 cells. The presence of AOF provided greater protective effects in the Dox environment. In addition, AOF‐pretreated ADMSCs showed enhanced migration than those treated with AOF in Dox environment. Therefore, our results show that administration of AOF preconditioned stem cells is potentially an effective strategy in the management of aging‐associated cardiac disorders. Abstract : Adipose‐derived mesenchymal stem cells (ADMSCs) preconditioned with Alpinate Oxyphyllae Fructus (AOF) protected H9c2 cells against doxorubicin (Dox) induced senescence by improving cell survival and longevity and by elevating the stem cell migration toward damaged H9c2 cells and upregulating the expression of homing‐associated C‐X‐C chemokine receptor type 7 (CXCR7).