Immune co-culture cell microarray – a feasible tool for high-throughput functional investigation of lymphocyte–cancer interactions. (1st January 2020)

• Record Type: Journal Article
• Authors: Baruch, Erez Nissim; Ortenberg, Rona; Avivi, Camila; Anafi, Liat; Dick-Necula, Daniela; Stossel, Chani; Moshkovits, Yonatan; Itzhaki, Orit; Besser, Michal Judith; Schachter, Jacob; Barshack, Iris; Markel, Gal
• Is Part Of: Oncoimmunology. Volume 9:Number 1(2020)
• Publication Date: 2020-01-01
• Subjects: Immunotherapy -- melanoma -- immunological cytotoxicity test -- functional protein expression -- omics validation; Tumors -- Immunological aspects -- Periodicals; Neoplasms -- therapy -- Periodicals; Immunotherapy -- Periodicals; 616.994
• DOI: 10.1080/2162402X.2020.1741267
• Languages: English
• ISSNs: 2162-402X

Abstract

ABSTRACT: Omics analyses often result in dozens to hundreds of potential targets, requiring validation for their biological relevance. Current high-throughput functional investigation methods are frequently labor-intensive, expensive, and display low reproducibility. The Immune Co-Culture Cell Microarray (ICCM) is a formalin-fixed paraffin-embedded cell block microarray based on co-cultures of patient-derived tumor-infiltrating lymphocytes and their autologous melanoma cells. Each ICCM slide represents the same experiment and can be stained using standard immunohistochemistry and immunofluorescence techniques. Functional dynamics assessment of both proteins and microRNAs using ICCM stained slides demonstrated similar findings to flow cytometry assays and to previously published patient-derived biopsy reports.