Phytochrome-Dependent Temperature Perception Modulates Isoprenoid Metabolism. Issue 3 (14th May 2020)

• Record Type: Journal Article
• Authors: Bianchetti, Ricardo; De Luca, Belen; de Haro, Luis A.; Rosado, Daniele; Demarco, Diego; Conte, Mariana; Bermudez, Luisa; Freschi, Luciano; Fernie, Alisdair R.; Michaelson, Louise V.; Haslam, Richard P.; Rossi, Magdalena; Carrari, Fernando
• Is Part Of: Plant physiology. Volume 183:Issue 3(2020)
• Page Start: 869
• Page End: 882
• Publication Date: 2020-05-14
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1104/pp.20.00019
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract : Phytochrome-mediated temperature perception compromises plastidial development and function, impairing isoprenoid metabolism in tomato leaves and fruits. Abstract: Changes in environmental temperature influence many aspects of plant metabolism; however, the underlying regulatory mechanisms remain poorly understood. In addition to their role in light perception, phytochromes (PHYs) have been recently recognized as temperature sensors affecting plant growth. In particular, in Arabidopsis ( Arabidopsis thaliana ), high temperature reversibly inactivates PHYB, reducing photomorphogenesis-dependent responses. Here, we show the role of phytochrome-dependent temperature perception in modulating the accumulation of isoprenoid-derived compounds in tomato ( Solanum lycopersicum ) leaves and fruits. The growth of tomato plants under contrasting temperature regimes revealed that high temperatures resulted in coordinated up-regulation of chlorophyll catabolic genes, impairment of chloroplast biogenesis, and reduction of carotenoid synthesis in leaves in a PHYB1B2-dependent manner. Furthermore, by assessing a triple phyAB1B2 mutant and fruit-specific PHYA - or PHYB2 -silenced plants, we demonstrated that biosynthesis of the major tomato fruit carotenoid, lycopene, is sensitive to fruit-localized PHY-dependent temperature perception. The collected data provide compelling evidence concerning the impact of PHY-mediated temperature perception on plastid metabolism in both leaves and fruit, specifically on the accumulation of isoprenoid-derived compounds.