Inflammatory molecules produced by meniscus and synovium in early and end‐stage osteoarthritis: a coculture study. Issue 7 (19th November 2018)

• Record Type: Journal Article
• Authors: Favero, Marta; Belluzzi, Elisa; Trisolino, Giovanni; Goldring, Mary B; Goldring, Steven R; Cigolotti, Augusto; Pozzuoli, Assunta; Ruggieri, Pietro; Ramonda, Roberta; Grigolo, Brunella; Punzi, Leonardo; Olivotto, Eleonora
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 7(2019:Jul.)
• Page Start: 11176
• Page End: 11187
• Publication Date: 2018-11-19
• Subjects: coculture -- inflammation -- knee -- meniscus -- osteoarthritis -- synovium; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.27766
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: The aim of this study was to identify the molecules and pathways involved in the cross‐talk between meniscus and synovium that may play a critical role in osteoarthritis (OA) pathophysiology. Samples of synovium and meniscus were collected from patients with early and end‐stage OA and cultured alone or cocultured. Cytokines, chemokines, metalloproteases, and their inhibitors were evaluated at the gene and protein levels. The extracellular matrix (ECM) changes were also investigated. In early OA cultures, higher levels of interleukin‐6 (IL‐6) and IL‐8 messenger RNA were expressed by synovium and meniscus in coculture compared with meniscus cultured alone. RANTES release was significantly increased when the two tissues were cocultured compared with meniscus cultured alone. Increased levels of matrix metalloproteinase‐3 (MMP‐3) and MMP‐10 proteins, as well as increased release of glycosaminoglycans and aggrecan CS846 epitope, were observed when synovium was cocultured with meniscus. In end‐stage OA cultures, increased levels of IL‐8 and monocyte chemoattractant protein‐1 (MCP‐1) proteins were released in cocultures compared with cultures of meniscus alone. Chemokine (C‐C motif) ligand 21 (CCL21) protein release was higher in meniscus cultured alone and in coculture compared with synovium cultured alone. Increased levels of MMP‐3 and 10 proteins were observed when tissues were cocultured compared with meniscus cultured alone. Aggrecan CS846 epitope release was increased in cocultures compared with cultures of either tissue cultured alone. Our study showed the production of inflammatory molecules by synovium and meniscus which could trigger inflammatory signals in early OA patients, and induce ECM loss in the progressive and final stages of OA pathology. Abstract : Inflammatory mediators, metalloproteinases, and metalloproteinases inhibitors are produced by meniscus and synovium from early and end‐stage osteoarthritic patients. Production of inflammatory molecules by synovium and meniscus can trigger inflammatory signals in early osteoarthritis (OA) patients, and induce extracellular matrix loss in the progressive and final stages of OA pathology. The interaction between synovium and meniscus is suggested in early and late osteoarthritic patients and it may provide a basis for identifying the mechanisms contributing to the development of OA associated with meniscal tear.