Ca2+‐dependent and Ca2+‐independent somatic release from trigeminal neurons. Issue 7 (7th December 2018)

• Record Type: Journal Article
• Authors: Sforna, Luigi; Franciolini, Fabio; Catacuzzeno, Luigi
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 7(2019:Jul.)
• Page Start: 10977
• Page End: 10989
• Publication Date: 2018-12-07
• Subjects: capacitance measurements -- somatic release -- trigeminal neurons -- voltage‐dependent calcium channels; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.27901
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Besides the nerve endings, the soma of trigeminal neurons also respond to membrane depolarizations with the release of neurotransmitters and neuromodulators in the extracellular space within the ganglion, a process potentially important for the cross‐communication between neighboring sensory neurons. In this study, we addressed the dependence of somatic release on Ca 2+ influx in trigeminal neurons and the involvement of the different types of voltage‐gated Ca 2+ (Cav) channels in the process. Similar to the closely related dorsal root ganglion neurons, we found two kinetically distinct components of somatic release, a faster component stimulated by voltage but independent of the Ca 2+ influx, and a slower component triggered by Ca 2+ influx. The Ca 2+ ‐dependent component was inhibited 80% by ω‐conotoxin‐MVIIC, an inhibitor of both N‐ and P/Q‐type Cav channels, and 55% by the P/Q‐type selective inhibitor ω‐agatoxin‐IVA. The selective L‐type Ca 2+ channel inhibitor nimodipine was instead without effect. These results suggest a major involvement of N‐ and P/Q‐, but not L‐type Cav channels in the somatic release of trigeminal neurons. Thus antinociceptive Cav channel antagonists acting on the N‐ and P/Q‐type channels may exert their function by also modulating the somatic release and cross‐communication between sensory neurons. Abstract : In this study we addressed the dependence of somatic release on Ca 2+ influx in trigeminal neurons, and the involvement of the different types of voltage‐gated Ca 2+ (Cav) channels in the process. We found two kinetically distinct components of somatic release, a faster component stimulated by voltage but independent of the Ca 2+ influx, and a slower component triggered by Ca 2+ influx though N‐ and P/Q‐type Cav channels.