Improved tumor tissue penetration and tumor cell uptake achieved by delayed charge reversal nanoparticles. (15th October 2017)

• Record Type: Journal Article
• Authors: Gou, Jingxin; Liang, Yuheng; Miao, Linlin; Guo, Wei; Chao, Yanhui; He, Haibing; Zhang, Yu; Yang, Jingyu; Wu, Chunfu; Yin, Tian; Wang, Yanjiao; Tang, Xing
• Is Part Of: Acta biomaterialia. Volume 62(2017)
• Page Start: 157
• Page End: 166
• Publication Date: 2017-10-15
• Subjects: Polyion complex -- Cabazitaxel -- Tumor penetration -- Nanoparticle -- Drug delivery -- Charge reversal; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1016/j.actbio.2017.08.025
• Languages: English
• ISSNs: 1742-7061

Abstract

Graphical abstract: Abstract: The high affinity of positively charged nanoparticles to biological interfaces makes them easily taken up by tumor cells but limits their tumor permeation due to non-specific electrostatic interactions. In this study, polyion complex coated nanoparticles with different charge reversal profiles were developed to study the influence of charge reversal profile on tumor penetration. The system was constructed by polyion complex coating using micelles composed of poly (lysine)- b -polycaprolactone (PLys- b -PCL) as the cationic core and poly (glutamic acid)- g - methoxyl poly (ethylene glycol) (PGlu- g -mPEG) as the anionic coating material. Manipulation of charge reversal profile was achieved by controlling the polymer chain entanglement and electrostatic interaction in the polyion complex layer through glutaraldehyde-induced shell-crosslinking. The delayed charge reversal nanoparticles (CTCL30) could maintain negatively charged in pH 6.5 PBS for at least 2 h and exhibit pH-responsive cytotoxicity and cellular uptake in an extended time scale. Compared with a faster charge reversal counterpart (CTCL70) with similar pharmacokinetic profile, CTCL30 showed deeper penetration, higher in vivo tumor cell uptake and stronger antitumor activity in vivo (tumor inhibition rate: 72.3% vs 60.2%, compared with CTCL70). These results indicate that the delayed charge reversal strategy could improve therapeutic effect via facilitating tumor penetration. Statement of Significance: Here, the high tumor penetration capability of PEG-coated nanoparticles and the high cellular uptake of cationic nanoparticles were combined by a delayed charge reversal drug delivery system. This drug delivery system was composed of a drug-loading cationic inner core and a polyion complex coating. Manipulation of charge reversal profile was realized by varying the crosslinking degree of the shell of the cationic inner core, through which changed the strength of the polyion complex layer. Nanoparticles with delayed charge reversal profile exhibited improved tumor penetration, in vivo tumor cell uptake and in vivo tumor growth inhibition effect although they have similar pharmacokinetic and biodistribution behaviors with their instant charge reversal counterpart.