High levels of Tryptophan reduce cell wall or membrane stress tolerance in Saccharomyces cerevisiae. Issue 10 (13th August 2021)

• Record Type: Journal Article
• Authors: Ohashi, Kazuto; Chaleckis, Romanas
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 85:Issue 10(2021)
• Page Start: 2131
• Page End: 2136
• Publication Date: 2021-08-13
• Subjects: Tryptophan -- Saccharomyces cerevisiae -- stress response; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1093/bbb/zbab142
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: Tryptophan (Trp) is a proteinogenic aromatic amino acid; however, high levels of Trp are toxic in animals and yeast with unknown mechanisms. Previously, we suggested that aromatic aminotransferase Aro9 is important for excess Trp degradation. Besides, Schroeder and Ikui showed that aro9Δ is sensitive to membrane stress by sodium dodecyl sulfate. Therefore, Trp accumulation may reduce the cell wall or membrane (CW/M) stress tolerance through participation of cell wall integrity (CWI) pathway, which detects and responds to CW/M perturbations. In this study, we found that yeast mutants of the CWI mitogen-activated protein kinase cascade were susceptible to excess Trp. Also, the Trp degradation deficient mutant aro8Δ aro9Δ cells, in which Trp accumulation was confirmed, were sensitive to several CW/M stresses. These results indicated that accumulation of Trp is adverse for the CW/M stress resistance and may disturb appropriate signal transduction responding to the stress. Graphical Abstract: