Functional Equivalency in Human Somatic Cell Nuclear Transfer-Derived Endothelial Cells. (22nd February 2019)

• Record Type: Journal Article
• Authors: Park, Soon-Jung; Lee, Ji-Heon; Lee, Seul-Gi; Lee, Jeoung Eun; Seo, Joseph; Choi, Jong Jin; Jung, Taek-Hee; Chung, Eun-Bin; Kim, Ha Na; Ju, Jongil; Song, Yun-Ho; Chung, Hyung-Min; Lee, Dong Ryul; Moon, Sung-Hwan
• Is Part Of: Stem cells. Volume 37:Number 5(2019)
• Page Start: 623
• Page End: 630
• Publication Date: 2019-02-22
• Subjects: Human somatic cell nuclear transfer -- Endothelial cell -- Ischemia -- Cell transplantation -- Cell therapy; Cloning -- Periodicals; Clone cells -- Periodicals; Stem cells -- Periodicals; Cell Differentiation -- Periodicals; Cell Division -- Periodicals; Clone Cells -- Periodicals; Hematopoietic Stem Cells -- Periodicals; Stem Cells -- Periodicals; 571.84
• DOI: 10.1002/stem.2986
• Languages: English
• ISSNs: 1066-5099

Abstract

Abstract : The derivation of human embryonic stem cells (hESCs) by somatic cell nuclear transfer (SCNT) has prompted a re-emerging interest in using such cells for therapeutic cloning. Despite recent advancements in derivation protocols, the functional potential of CHA-NT4 derived cells is yet to be elucidated. For this reason, this study sought to differentiate CHA-NT4 cells toward an endothelial lineage in order to evaluate in vitro and in vivo functionality. To initial differentiation, embryoid body formation of CHA-NT4 was mediated by concave microwell system which was optimized for hESC-endothelial cell (EC) differentiation. The isolated CD31+ cells exhibited hallmark endothelial characteristics in terms of morphology, tubule formation, and ac-LDL uptake. Furthermore, CHA-NT4-derived EC (human nuclear transfer [hNT]-ESC-EC) transplantation in hind limb ischemic mice rescued the hind limb and restored blood perfusion. These findings suggest that hNT-ESC-EC are functionally equivalent to hESC-ECs, warranting further study of CHA-NT4 derivatives in comparison to other well established pluripotent stem cell lines. This revival of human SCNT-ESC research may lead to interesting insights into cellular behavior in relation to donor profile, mitochondrial DNA, and oocyte quality. Stem Cells 2019;37:623–630 : Abstract : NT-ESC derived ECs through a concave microwell system demonstrates functional potential regarding the restoration of blood perfusion to an ischemic limb through angiogenic factors.