Curcumin primed exosomes reverses LPS‐induced pro‐inflammatory gene expression in buffalo granulosa cells. Issue 2 (28th August 2017)

• Record Type: Journal Article
• Authors: Vashisht, Monika; Rani, Payal; Sunita, ; Onteru, Suneel Kumar; Singh, Dheer
• Is Part Of: Journal of cellular biochemistry. Volume 119:Issue 2(2018)
• Page Start: 1488
• Page End: 1500
• Publication Date: 2017-08-28
• Subjects: DLS(dynamic light scattering) -- ELISA (enzyme linked immuno sorbent assay) -- fluorescent labeling -- reprogramming -- toll like receptor -- viability; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.26309
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: Curcumin possesses anti‐inflammatory properties and provides a promising treatment for inflammation. The aim of the study is to establish that buffalo granulosa cells when primed with curcumin (20 μM), release improved cellular contents through exosome that can mitigate granulosa cell dysfunction. Recently, we have shown that buffalo granulosa cells exposed to LPS (1 μg/mL) in serum free culture, transiently increased the pro‐inflammatory cytokine genes (IL‐1β, TNF‐α, IL‐6) expression followed by the inhibition of CYP19A1 gene expression and estradiol production. Therefore, LPS‐treated granulosa cells were used as a model of inflammation and curcumin primed exosomes were utilized to check their potential for reducing granulosa cell dysfunction. Expression level of pro‐inflammatory cytokines and CYP19A1 were detected by real time PCR while estradiol levels were measured by ELISA. Exosomes derived from curcumin‐treated cells alleviated LPS mediated inflammation. In conclusion, our study potentiates the use of curcumin primed exosomes in mitigating granulosa cell dysfunction. Results show the therapeutic conservatories of curcumin via primed exosomes. Abstract : Schematic representation of the methodology and concept representing the reprogramming of exosomes after curcumin treatment in granulosa cells. Curcumin primed exosomes decreases the inflammation and improves fertility in LPS induced granulosa cells.