Isolation of peste des petits ruminants virus using primary goat kidney cell culture from kidneys obtained at slaughter. Issue 3 (16th December 2020)

• Record Type: Journal Article
• Authors: Begum, Shahana; Nooruzzaman, Mohammed; Hasnat, Azmary; Parvin, Mst. Murshida; Parvin, Rokshana; Islam, Mohammad Rafiqul; Chowdhury, Emdadul Haque
• Is Part Of: Veterinary medicine and science. Volume 7:Issue 3(2021)
• Page Start: 915
• Page End: 922
• Publication Date: 2020-12-16
• Subjects: Black Bengal goats -- goat kidney cell culture -- peste des petits ruminants -- virus isolation; Veterinary medicine -- Periodicals; Animal Diseases; Veterinary medicine; Periodicals; Periodicals; 636.08905
• DOI: 10.1002/vms3.413
• Languages: English
• ISSNs: 2053-1095

Abstract

Abstract: Background: Traditionally isolation of peste des petits ruminant virus (PPRV) is performed in Vero cells that takes several blind passages before observing typical cytopathic effects (CPEs). As an alternate, researchers have been using lamb kidney (LK) cells but day‐old lambs are difficult to obtain and requires animal sacrifice. Objective: We established a primary goat kidney (GK) cell culture from the kidneys obtained at slaughter. Methods: The kidney of Black Bengal goats were collected from slaughter house and processed to make single cell suspension. The cells were resuspended in appropriate culture medium and maintained under optimum culture condition. Results: The 80% confluent monolayer of GK cells was obtained after 15–20 days post seeding. Upon infection with a field isolate of PPRV, the well‐developed CPEs characterized by cell rounding, vacuolation in the cytoplasm and fusion of cells were observed after 48 hr post infection. Virus quantification in the culture supernatant revealed more viral RNA in GK cells than LK cells. The multicycle growth analysis of PPRV showed a steady increase in the virus loads in the culture supernatant of infected GK cells, suggesting an adaptation of the PPRV in GK cells. Conclusions: The findings suggest that primary GK cells can be successfully prepared from the mature kidney cortical tissues and can be used for the isolation of PPRV. This system could reduce the unnecessary sacrifice of lambs or kids. Since kidneys of slaughtered goats are available throughout the year, using this protocol primary cell culture from mature goat kidney can provide primary cells to the laboratory throughout the year. Abstract : We established a primary goat kidney cell culture system using kidneys of adult goats obtained at slaughter. The developed cell culture system allows efficient replication of field strain of peste des petits ruminants virus (PPRV). Therefore, primary goat kidney cells can be successfully prepared from the mature kidney cortical tissues and can be used for the isolation of PPRV.