Bone marrow stromal cells transplantation promotes the resolution and recanalization of deep vein thrombosis in rabbits through regulating macrophage infiltration and angiogenesis. Issue 7 (20th February 2019)

• Record Type: Journal Article
• Authors: Zhang, Jingyong; Kong, Xiangqian; Jin, Xing; Gao, Peixian; Wang, Maohua; Yang, Le
• Is Part Of: Journal of cellular biochemistry. Volume 120:Issue 7(2019)
• Page Start: 11680
• Page End: 11689
• Publication Date: 2019-02-20
• Subjects: angiogenesis -- bone marrow stromal cells transplantation -- deep vein thrombosis -- macrophage infiltration -- rabbits; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.28447
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: This study aims to validate whether bone marrow stromal cells (BMSCs) transplantation could promote the resolution and recanalization of deep vein thrombosis (DVT) and to explore the underlying mechanism. The right hind femoral vein was embolized to establish the DVT rabbit model. BMSCs from New Zealand white rabbits were isolated and identified, and then injected into DVT rabbits. After that, the extent of angiogenesis was determined by the amount of capillaries that were positive for antibody against vWF. Macrophage infiltration was measured by immunohistochemistry with F4/80 antibody. M1 or M2 macrophages were identified as F4/80 + CD11c + or F4/80 + CD206 + cells by using flow cytometry analysis, respectively. BMSCs were successfully isolated and identified. BMSCs transplantation promotes macrophage infiltration and angiogenesis in DVT rabbits. BMSCs transplantation causes M1/M2 polarization, altered cytokine production and increased monocyte chemotactic protein 1 (MCP‐1) protein expression in DVT rabbits. However, injection of MCP‐1 protein not only reversed the effects of BMSCs transplantation on macrophage infiltration and angiogenesis, but also reversed the effects of BMSCs transplantation on M1/M2 polarization and cytokine production in DVT rabbits. BMSCs transplantation promotes the resolution and recanalization of DVT in rabbits through regulating macrophage infiltration and angiogenesis, the underlying mechanism is associated with MCP‐1 expression. Abstract : Our study indicated that bone marrow stromal cells transplantation promotes the resolution and recanalization of deep vein thrombosis in rabbits through regulating macrophage infiltration and angiogenesis, one of the underlying mechanisms is partially associated with monocyte chemotactic protein 1 expression.