An immunosuppressive peptide from the horsefly inhibits inflammation by repressing macrophage maturation and phagocytosis. Issue 8 (11th April 2019)

• Record Type: Journal Article
• Authors: Chen, Ran; Yuan, Long; Cao, Nengqi; Li, Pengpeng; Chen, Huilin; Zhou, Jiaxin; Hao, Xue; Liu, Tong; Yang, Wen‐Hao; Cui, Shuzhong; Yan, Xiuwen
• Is Part Of: Journal of cellular biochemistry. Volume 120:Issue 8(2019)
• Page Start: 14116
• Page End: 14126
• Publication Date: 2019-04-11
• Subjects: anti‐inflammation -- ectoparasite -- Hybomitra atriperoides -- immunoregulin HA -- macrophage -- peptide; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.28687
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: Ectoparasites repress host immune responses while they obtain nutrition from their hosts. Understanding the immunosuppressive mechanisms between ectoparasites and their hosts will provide new strategies to develop potential immunosuppressive drugs against immune disorder diseases. Previously, we have discovered that a small peptide, immunoregulin HA, from the horsefly ( Hybomitra atriperoides ) may play an immunosuppressive role in rat splenocytes. However, the targeting cells and detailed mechanisms of immunoregulin HA in immunosuppressive reactions are not well defined. Here, we show that immunoregulin HA reduces the secretion of proinflammatory cytokines upon lipopolysaccharide (LPS) stimulation. Interestingly, we discover that the major cytokines repressed by immunoregulin HA are secreted by macrophages, rather than by T cells. Furthermore, immunoregulin HA inhibits macrophage maturation and phagocytosis. Mechanically, the activations of c‐JUN N‐terminal kinase and extracellular signal‐regulated kinase upon LPS stimulation are decreased by immunoregulin HA. Consistently, immunoregulin HA treatment exhibits an anti‐inflammatory activity in a mouse model of adjuvant‐induced paw inflammation. Taken together, our data reveal that immunoregulin HA conducts the anti‐inflammatory activity by blocking macrophage functions. Abstract : Our findings suggest that the anti‐inflammatory peptide, immunoregulin HA form the salivary gland of the horsefly, may be a potential drug for the treatment of macrophage activation syndrome and offer insights into the ectoparasite‐host relationship.