Experimental study on the antioxidant activity of Malus hupehensis (Pamp.) Rehd extracts in vitro and in vivo. Issue 7 (19th February 2019)

• Record Type: Journal Article
• Authors: Liu, Ji; Guo, Dongyan; Fan, Yu; Sun, Jing; Cheng, Jiangxue; Shi, Yajun
• Is Part Of: Journal of cellular biochemistry. Volume 120:Issue 7(2019)
• Page Start: 11878
• Page End: 11889
• Publication Date: 2019-02-19
• Subjects: antiaging -- antioxidant -- d‐galactose -- Malus hupehensis (Pamp.) Rehd extracts -- subacute aging; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.28469
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: Extracts of Malus hupehensis (Pamp.) Rehder, containing flavonoids with good antioxidant and antiliver injury properties, possess various biological activities. The aim of this study was to explore the antioxidant activity of these extracts in vitro and in vivo. The antioxidant activity of the extracts was studied using scavenging 1, 1‐diphenyl‐2‐picrylhydrazyl (DPPH) free radicals, 2, 2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) radicals, and superoxide free radicals and by inhibiting mushroom tyrosinase activity in vitro. An in vivo antioxidant experiment was performed using a rat‐aging model. Aging was induced in rats with D‐galactose through treating them at doses of extracts about 150, 300, and 600 mg·kg −1 ·day −1 . The Malus hupehensis extracts showed high antioxidant activity; the IC50 values of DPPH radicals, ABTS radicals, superoxide radicals, and mushroom tyrosinase inhibition were 19.00 μg/mL, 303.94 μg/mL, and 3.71 mg/mL, and 1.16 mg/mL, respectively. Our experiments showed that the extracts significantly increased the activity of antioxidant enzymes in the serum and tissue homogenate in vivo, and that the effects were positively correlated with the dose, compared with the activity observed in controls. Histopathological observation also confirmed that the extracts had protective effects after oxidative injury in rat tissues. In conclusion, the extracts of M. hupehensis showed effective antioxidant activity both in vitro and in vivo. Abstract : Malus hupehensis extracts, containing a class of naturally occurring flavonoids, have good DPPH, ABTS, and superoxide free radical scavenging effects and inhibit tyrosinase activity in vitro. In addition, M. hupehensis extracts can increase the levels of SOD, CAT, GSH‐Px, and T‐AOC and decrease the contents of AST, ALT and MDA, as well as play an effective protective role in organ injury in vivo.