Interaction of barley β-glucan with food dye molecules – An insight from pulse dipolar EPR spectroscopy. (1st June 2023)

• Record Type: Journal Article
• Authors: Wu, Xiaowen; Boulos, Samy; Syryamina, Victoria; Nyström, Laura; Yulikov, Maxim
• Is Part Of: Carbohydrate polymers. Volume 309(2023)
• Publication Date: 2023-06-01
• Subjects: Water-soluble dietary fibers -- β-Glucan -- Binding interaction -- Food dye -- Electron paramagnetic resonance, double electron-electron resonance; DF dietary fiber -- H-bond hydrogen bond -- EPR electron paramagnetic resonance -- ADHD attention deficit hyperactivity disorder -- BG β-glucan -- DEER double electron-electron resonance -- ED-EPR echo detected EPR -- SL-BG spin-labelled BG -- CalW calcofluor white -- ConR congo red -- BB brilliant blue -- PB patent blue -- BBlc brilliant black -- CA carminic acid -- SSE sum stretched exponential function -- ESEEM electron spin echo envelope modulation -- rmsd root-mean-square deviation; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2023.120698
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: The interactions between dietary fibers (DFs) and small molecules are of great interest to food chemistry and nutrition science. However, the corresponding interaction mechanisms and structural rearrangements of DFs at the molecular level are still opaque due to the usually weak binding and the lack of appropriate techniques to determine details of conformational distributions in such weakly organized systems. By combining our previously established methodology on stochastic spin-labelling of DFs with the appropriately revised set of pulse electron paramagnetic resonance techniques, we present here a toolkit to determine the interactions between DFs and small molecules, using barley β-glucan as an example for neutral DF and a selection of food dye molecules as examples for small molecules. The proposed here methodology allowed us to observe subtle conformational changes of β-glucan by detecting multiple details of the local environment of the spin labels. Substantial variations of binding propensities were detected for different food dyes. Graphical abstract: Unlabelled Image