Melatonin effective to reduce the microscopic symptoms of polycystic ovary syndrome-related infertility: An experimental study. (April 2023)

• Record Type: Journal Article
• Authors: Arık, Gökçe Nur; Kaplanoğlu, Gülnur Take; Sağlam, Atiye Seda Yar; Elmazoğlu, Zübeyir; Dinçel, Aylin Sepici; Seymen, Cemile Merve
• Is Part Of: Tissue & cell. Volume 81(2023)
• Publication Date: 2023-04
• Subjects: Polycystic ovary syndrome -- Melatonin -- MT1 receptor -- GDF9 -- BMP15; Cytology -- Periodicals; 571.5
• DOI: 10.1016/j.tice.2023.102015
• Languages: English
• ISSNs: 0040-8166

Abstract

Abstract: Polycystic ovary syndrome (PCOS) is an endocrine disorder seen in women of reproductive age and has been gradually increasing over the years. The mechanism of the syndrome has still not been clearly understood. In this study, the possible effects of exogenously administrated melatonin on melatonin (MT1) receptor, Growth Differentiation Factor-9 (GDF9), and Bone Morphogenetic Protein-15 (BMP15) in experimental PCOS were investigated. Thirty-two 6–8-week-old Sprague-Dawley rats were randomly divided into four groups ( n = 8 in each) as Sham control (Group 1), Melatonin (Group 2), PCOS (Group 3), and PCOS + Melatonin (Group 4) groups. At the end of the 21st day, the experiment was terminated, the ovary tissues were taken, and Hematoxylin-Eosin staining, MT1, GDF9, BMP15 immunohistochemical labeling, western blot, and quantitative real-time polymerase chain reaction (qPCR) analyses were performed. Serum Luteinizing Hormone (LH)/Follicle Stimulating Hormone (FSH) levels and colpo-cytological examinations were also carried out. The results revealed that melatonin administration increased the expression levels of the MT1 receptor, GDF9, and BMP15 in PCOS at protein and mRNA levels. It was determined that melatonin administration reduced the microscopic symptoms of PCOS. Melatonin was found to be effective via the MT1 receptor in the pathogenesis of PCOS, and it suppressed the transport pathways of GDF9 to granulosa cells in antral follicles. Highlights: Melatonin increased MT1R, GDF9 and BMP15 levels in PCOS. Melatonin administrations reduced the micrscopic symptoms of PCOS. Melatonin was found to be effective in the PCOS pathogenesis via MT1R. Melatonin suppress the transport pathways of GDF9 to granulosa cells in antral follicles.