IL-4/13 expressing CD3γ/δ+ T cells regulate mucosal immunity in response to Flavobacterium columnare infection in grass carp. Issue 134 (March 2023)

• Record Type: Journal Article
• Authors: Xiao, Hehe; Yun, Shengran; Huang, Wenji; Dang, Huifeng; Jia, Zhao; Chen, Kangyong; Zhao, Xin; Wu, Yaxin; Shi, Yanjie; Wang, Junya; Zou, Jun
• Is Part Of: Fish & shellfish immunology. Issue 134(2023)
• Publication Date: 2023-03
• Subjects: IL-4/13 -- CD3 -- Monoclonal antibody -- Flavobacterium columnare -- Grass carp; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2023.108586
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Interleukin (IL) 4 and 13 are signature cytokines orchestrating Th2 immune response. Teleost fish have two homologs, termed IL-4/13A and IL-4/13B, and have been functionally characterized. However, what cells express IL-4/13A and IL-4/13B has not been investigated in fish. In this work, the recombinant IL-4/13A and IL-4/13B proteins of grass carp ( Ctenopharyngodon idella ) were produced in the Escherichia coli ( E. coli ) cells and purified. Monoclonal antibodies (mAbs) against the recombinant Ci IL-4/13A and Ci IL-4/13B proteins were prepared and characterized. Western blotting analysis showed that the Ci IL-4/13A and Ci IL-4/13B mAbs could specifically recognize the recombinant proteins expressed in the E. coli cells and HEK293T cells and did not cross-react with each other. Confocal microscopy revealed that the Ci IL-4/13A + and Ci IL-4/13B + cells were present in the gills, intestine and spleen and could be upregulated in fish infected with Flavobacterium columnare ( F. columnare ). Interestingly, the cells expressing Ci IL-4/13A and Ci IL-4/13B were mostly CD3γ/δ + cells. The CD3γ/δ + /IL-4/13A + and CD3γ/δ + /IL-4/13B + cells were significantly upregulated in the gill filaments and the intestinal mucosa after F. columnare infection. Our results imply that the CD3γ/δ + /IL-4/13A + and CD3γ/δ + /IL-4/13B + cells are important for homeostasis and the regulation of mucosal immunity. Highlights: Monoclonal antibodies against grass carp IL-4/13 were produced. The IL-4/13A + and IL-4/13B + cells were abundant in the gills and upregulated by F. columnare . The IL-4/13 expressing CD3γ/δ + cells were upregulated in the gills and intestine by F. columnare .