Extracellular biomineralization of uranium and its toxicity alleviation to Bacillus thuringiensis X-27. (May 2023)

• Record Type: Journal Article
• Authors: Zhu, Ting; Zeng, Qian; Zhao, Changsong; Wen, Yufeng; Li, Shangqing; Li, Feize; Lan, Tu; Yang, Yuanyou; Liu, Ning; Sun, Qun; Liao, Jiali
• Is Part Of: Journal of environmental radioactivity. Volume 261(2023)
• Publication Date: 2023-05
• Subjects: Uranium -- Bacillus thuringiensis -- Phosphate biomineralization -- Uramphite; Radioactivity -- Periodicals; Radiation, Background -- Periodicals; Radioecology -- Periodicals; Radioactive pollution -- Periodicals; Environmental Pollutants -- Periodicals; Radioactive Pollutants -- Periodicals; Radioactivity -- Periodicals; Radioécologie -- Périodiques; Pollution radioactive -- Périodiques; Fond de rayonnement -- Périodiques; 539.752
• DOI: 10.1016/j.jenvrad.2023.107126
• Languages: English
• ISSNs: 0265-931X

Abstract

Abstract: Uranium biomineralization can slow uranium migration in the environment and thus prevent it from further contaminating the surroundings. Investigations into the uranium species, pH, inorganic phosphate (Pi) concentration, and microbial viability during biomineralization by microorganisms are crucial for understanding the mineralization mechanism. In this study, Bacillus thuringiensis X-27 was isolated from soil contaminated with uranium and was used to investigate the formation process of uranium biominerals induced by X-27. The results showed that as biomineralization proceeded, amorphous uranium-containing deposits were generated and transformed into crystalline minerals outside cells, increasing the overall concentration of uramphite. This is a cumulative rather than abrupt process. Notably, B. thuringiensis X-27 precipitated uranium outside the cell surface within 0.5 h, while the release of Pi into the extracellular environment and the change of pH to alkalescence further promoted the formation of uramphite. In addition, cell viability determination showed that the U(VI) biomineralization induced by B. thuringiensis X-27 was instrumental in alleviating the toxicity of U(VI) to cells. This work offers insight into the mechanism of U(VI) phosphate biomineralization and is a reference for bioremediation-related studies. Highlights: Bacillus thuringiensis X-27 precipitated U rapidly to uramphite outside cells, which can alleviate U toxicity to cells.