A near infrared fluorescent probe for rapid sensing of peroxynitrite in living cells and breast cancer mice. Issue 12 (14th March 2023)

• Record Type: Journal Article
• Authors: Xu, Zixiang; Xu, Zhencai; Zhang, Dong
• Is Part Of: RSC advances. Volume 13:Issue 12(2023)
• Page Start: 8262
• Page End: 8269
• Publication Date: 2023-03-14
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d3ra01024d
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Peroxynitrite (ONOO − ) plays an essential role in numerous physiological and pathological processes owing to its strong oxidation and nitrification. Abstract : Peroxynitrite (ONOO − ) plays an essential role in numerous physiological and pathological processes owing to its strong oxidation and nitrification. Many studies have shown that ONOO − abnormalities are associated with inflammatory diseases, even cancer, such as arthritis, hepatitis, pneumonia, and breast cancer. Thus, developing a trustworthy technology to monitor ONOO − levels is critical in inflammatory or cancer illnesses. Herein, an ultrafast near-infrared (NIR) fluorescent probe (Cy–OH–ONOO) is proposed to detect ONOO − within 30 s. The probe's borate moiety is oxidized and separated from Cy–OH–ONOO, releasing a NIR fluorescence signal after interacting with ONOO − under physiological circumstances. In addition, the probe displays good selectivity and sensitivity towards ONOO − compared to other related biological species. Moreover, it is applied to the image and detects the level fluctuation of ONOO − in living cells and breast cancer mice based on excellent features with high biocompatibility and low toxicity of the developed probe. Therefore, Cy–OH–ONOO could serve as a powerful imaging tool to understand the correlation of ONOO − with inflammatory or breast cancer pathophysiological processes and to assess ONOO − levels in cellular oxidative stress.