Light induced diversity-oriented synthesis (DOS) library of annulated indolizine fluorophores for imaging non-lysosomal lipid droplets (LDs). Issue 10 (13th February 2023)

• Record Type: Journal Article
• Authors: Maiti, Debajit; Munan, Subrata; Singh, Shweta; Das, Ranajit; Samanta, Animesh; Sen, Subhabrata
• Is Part Of: Journal of materials chemistry. Volume 11:Issue 10(2023)
• Page Start: 2191
• Page End: 2199
• Publication Date: 2023-02-13
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/d2tb02656b
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : DASS fluor (a novel annulated indolizines based fluorescent probe): their synthesis via DOS and biological evaluation for non-lysosomal LD detection. Abstract : We report the design, synthesis, and biological evaluation of a novel class of annulated indolizines as fluorescent probes. The compounds were generated through an eco-friendly, blue LED-induced domino reaction in ethyl acetate. A library of 24 coloured compounds exhibited tuneable emissions. One of the compounds (which we call DASS-fluor) proved to be an excellent polarity sensing probe. It is biocompatible, photostable, and detects specific types of lipid droplets (LDs in response to oleic acid, stress, and drug-induced autophagy in lungs and hepatic carcinoma cells). In comparison to Nile Red (a commercial probe), DASS-fluor can differentiate non-lysosomal LDs from lysosomal LDs and offers an advantage in precisely mapping drug-induced lipidosis caused by increased non-lysosomal LDs in cancerous cells. This unique probe could be a potential fluorescent marker for specific types of lipidosis induced by drugs.