(Photo-)crosslinkable gelatin derivatives for biofabrication applications. (1st October 2019)

• Record Type: Journal Article
• Authors: Van Hoorick, Jasper; Tytgat, Liesbeth; Dobos, Agnes; Ottevaere, Heidi; Van Erps, Jürgen; Thienpont, Hugo; Ovsianikov, Aleksandr; Dubruel, Peter; Van Vlierberghe, Sandra
• Is Part Of: Acta biomaterialia. Volume 97(2019)
• Page Start: 46
• Page End: 73
• Publication Date: 2019-10-01
• Subjects: 1H-NMR proton-nuclear magnetic resonance spectroscopy -- 2PP two-photon polymerization -- Ala alanine -- BMSC Bone marrow Stromal Cells -- CAD computer aided design -- Cys cysteine -- DAS tetrapotassium 4, 4′-(1, 2-ethenediyl)bis(2-(3-sulfo-phenyl)diazenesulfonate) -- DBA diisobutylacrylamide -- DLP digital light projection -- DMD digital micromirror device -- DS degree of substitution -- DSC differential scanning calorimetry -- DTP 3, 3′-dithiobis(propionic hydrazide) -- DTT dithiothreitol -- ECM extracellular matrix -- FI furfuryl isocyanate -- FA furfuryl amine -- G′ storage modulus -- G″ Loss modulus -- GAGs glycosaminoglycans -- gel-AA gelatin-acrylamide -- gel-AC gelatin-anthracene -- gel-AGE gelatin allylglycidyl ether -- gelatin-cys gelatin-cysteine -- gelatin-Cys-2-MPD gelatin modified with cysteine and 2-mercaptopyrimidine-4, 6 diol -- gelatin-FA gelatin furfuryl amine -- gelatin-FI gelatin furfuryl isocyanate -- gelatin-PEG gelatin modified with poly(ethylene) glycol acrylate -- gelatin-TBA-MNA gelatin-thiobutylamidine modified with 2-mercaptonicotinic acid (MNA) -- gelatin-tyramine gelatin modified with tyramine -- gelatin/tyramine/heparin gelatin modified with tyramine and heparin -- gel-boc-AEMA gelatin with boc protected primary amines and aminoethylmethacrylate coupled to the carboxylic acids -- gel-BTHE gelatin-3, 3′, 4, 4′-benzophenone tetra carboxylic dihydroxyethylmethacrylate -- gel-FGE gelatin furfuryl glycidyl ether -- gel-MA gelatin-methacrylamide -- gelMA-DA gelatin-methacrylamide-dopamine -- gel-MFVF gelatin-5-(2-(5-methyl furylene vinylene)) furancarboxyaldehyde -- gel-MOD gelatin-methacryloyl -- gel-MOD-AEMA gelatin-methacryloyl-aminoethylmethacrylate -- gel-NB gelatin-norbornene -- gel-NC gelatin-nitrocinnamate -- gel-PEG-cys gelatin-poly(ethylene glycol) cysteine -- gel-S thiolated gelatin -- gel-T gelatin tetrazine -- gel-VE gelatin-vinylester -- Gln glutamine -- Gly glycine -- GMA gelatin-methacryloyl-acyl -- Hν UV irradiation -- HRP horseradish peroxidase -- Irgacure 2959 (2-hydroxy-1-(4-(hydroxyethoxy)-phenyl)-2-methyl-1-propanone) -- Ile isoleucine -- LAP lithium phenyl-2, 4, 6-trimethylbenzoylphosphinate -- MPG methacrylated poly(ethylene glycol)-modified gelatin -- MFVF 5-(2-(5-methyl furylene vinylene))furancarboxyaldehyde -- MMP-1 matrix metalloproteinase -- MSC mesenchymal stem cell -- NHS n-hydroxysuccinimide -- NMR nuclear magnetic resonance spectroscopy -- P2CK sodium 3, 3′-(((1E, 1E′)-(2-oxocyclopentane-1, 3-diylidene) bis (methanylydiebe))bis(4, 1-phenylene))bis(methylazanediyl))dipropanoate -- P3-A tripropylene glycol diacrylate -- PBS phosphate buffered saline -- PEG poly(ethylene glycol) -- PEGDA poly(ethylene glycol) di acrylate -- PEGdNB poly(ethylene glycol) dinorbornene -- PEG4NB 4-arm poly(ethylene glycol)-norbornene -- PEG4SH 4-arm poly(ethylene glycol)-tetra thiol -- PEGTA 4-arm poly(ethylene glycol) tetra acrylate -- PI photoinitiator -- PPA dipentaerythritol pentaacrylate -- Pro proline -- PTA pentaerythritol triacrylate -- PVA poly(vinyl alcohol) -- PVA-MA polyvinylalcohol-methacrylate -- Ru/SPS photoinitiator based on a ruthenium complex (tris-bipyridyl-ruthenium (II) hexahydrate) and sodium persulfate (SPS) -- SLA stereolithography -- SPS sodium persulfate -- Td dissociation temperature -- Tg glass transition temperature -- TTA trimethylolpropane triacrylate -- TNBSA 2, 4, 6-trinitrobenzene sulfonic acid -- UDMA urethane-dimethacrylate -- UV Ultraviolet -- κ-carrageenan-MA kappa-carrageenan-methacrylate; Gelatin derivatives -- Crosslinking chemistry -- Modification strategies -- Additive manufacturing -- Biofabrication; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1016/j.actbio.2019.07.035
• Languages: English
• ISSNs: 1742-7061

Abstract

Graphical abstract: Abstract: Over the recent decades gelatin has proven to be very suitable as an extracellular matrix mimic for biofabrication and tissue engineering applications. However, gelatin is prone to dissolution at typical cell culture conditions and is therefore often chemically modified to introduce (photo-)crosslinkable functionalities. These modifications allow to tune the material properties of gelatin, making it suitable for a wide range of biofabrication techniques both as a bioink and as a biomaterial ink (component). The present review provides a non-exhaustive overview of the different reported gelatin modification strategies to yield crosslinkable materials that can be used to form hydrogels suitable for biofabrication applications. The different crosslinking chemistries are discussed and classified according to their mechanism including chain-growth and step-growth polymerization. The step-growth polymerization mechanisms are further classified based on the specific chemistry including different (photo-)click chemistries and reversible systems. The benefits and drawbacks of each chemistry are also briefly discussed. Furthermore, focus is placed on different biofabrication strategies using either inkjet, deposition or light-based additive manufacturing techniques, and the applications of the obtained 3D constructs. Statement of Significance: Gelatin and more specifically gelatin-methacryloyl has emerged to become one of the gold standard materials as an extracellular matrix mimic in the field of biofabrication. However, also other modification strategies have been elaborated to take advantage of a plethora of crosslinking chemistries. Therefore, a review paper focusing on the different modification strategies and processing of gelatin is presented. Particular attention is paid to the underlying chemistry along with the benefits and drawbacks of each type of crosslinking chemistry. The different strategies were classified based on their basic crosslinking mechanism including chain- or step-growth polymerization. Within the step-growth classification, a further distinction is made between click chemistries as well as other strategies. The influence of these modifications on the physical gelation and processing conditions including mechanical properties is presented. Additionally, substantial attention is put to the applied photoinitiators and the different biofabrication technologies including inkjet, deposition or light-based technologies.