Tetraselmis suecica biofilm cell destruction by high-pressure homogenization for protein extraction. (February 2023)

• Record Type: Journal Article
• Authors: Delran, Pauline; Frances, Christine; Guihéneuf, Freddy; Peydecastaing, Jérôme; Pontalier, Pierre-Yves; Barthe, Laurie
• Is Part Of: Bioresource technology reports. Volume 21(2023)
• Publication Date: 2023-02
• Subjects: Microalgae -- Mechanical and physical processes -- Cell lysis -- Biorefinery -- Green extraction; Biomass energy -- Periodicals; Biotransformation (Metabolism) -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Waste products as fuel -- Periodicals; Waste products as fuel; Organic wastes; Factory and trade waste; Biotransformation (Metabolism); Biomass energy; Agricultural wastes; Periodicals; Electronic journals; 662.88
• DOI: 10.1016/j.biteb.2023.101372
• Languages: English
• ISSNs: 2589-014X

Abstract

Abstract: The efficiency of cell destruction and extraction of intracellular contents by a high-pressure homogenizer was investigated on the Tetraselmis suecica microalgae to determine the possibility of integrating it into a biorefinery unit. Different process parameters relating to the use of a high-pressure homogenizer and biomass conditioning were tested. The parameter that has the greatest effect on cell destruction is pressure. From 40 MPa the maximum extraction yields of total proteins (80 %) and total carbohydrates (60 %) are reached and beyond 50 MPa, excessive cell destruction occurs, leading to the formation of aggregates. One single pass is sufficient to recover the maximal protein yield. Extraction in an alkaline solvent does not have a significant positive effect. Finally, it is possible to work in a wide range of biomass concentrations (from 10 g·L −1 to 100 g·L −1 ) and with fresh or after freeze-thaw cycles without significantly altering the yields. Graphical abstract: Unlabelled Image Highlights: HPH is an efficient mechanical cell destruction technique for protein extraction. A pressure of 10 MPa permeabilizes the cells and extracts a high protein yield. The destruction and disintegration of cells requires higher pressures. Multiple passes do not improve the product yield. The initial concentration and the solvent nature have no effect on the extraction.