Sulfation Pattern Dependent Iron(III) Mediated Interleukin‐8 Glycan Binding. (9th December 2021)

• Record Type: Journal Article
• Authors: Brunori, Francesco; Padhi, Deepak Kumar; Alshanski, Israel; Freyse, Joanna; Dürig, Jan‐Niklas; Penk, Anja; Vaccaro, Luigi; Hurevich, Mattan; Rademann, Jörg; Yitzchaik, Shlomo
• Is Part Of: Chembiochem. Volume 23:Number 3(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-12-09
• Subjects: electrochemistry -- interactions -- interleukin-8 -- metal binding -- sulfation pattern; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.202100552
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: Cytokines such as interleukin‐8 activate the immune system during infection and interact with sulfated glycosaminoglycans with specific sulfation patterns. In some cases, these interactions are mediated by metal ion binding which can be used to tune surface‐based glycan‐protein interactions. We evaluated the effect of both hyaluronan sulfation degree and Fe 3+ on interleukin‐8 binding by electrochemical impedance spectroscopy and surface characterizations. Our results show that sulfation degree and metal ion interactions have a synergistic effect in tuning the electrochemical response of the glycated surfaces to the cytokine. Abstract : The synergistic effect of hyaluronan sulfation degree and metal ion on interleukin‐8 binding was investigated by electrochemical impedance spectroscopy and surface characterizations.