CamelliA-based simultaneous imaging of Ca2+ dynamics in subcellular compartments. Issue 4 (26th February 2022)

• Record Type: Journal Article
• Authors: Guo, Jingzhe; He, Jiangman; Dehesh, Katayoon; Cui, Xinping; Yang, Zhenbiao
• Is Part Of: Plant physiology. Volume 188:Issue 4(2022)
• Page Start: 2253
• Page End: 2271
• Publication Date: 2022-02-26
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1093/plphys/kiac020
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract: As a universal second messenger, calcium (Ca 2+ ) transmits specific cellular signals via a spatiotemporal signature generated from its extracellular source and internal stores. Our knowledge of the mechanisms underlying the generation of a Ca 2+ signature is hampered by limited tools for simultaneously monitoring dynamic Ca 2+ levels in multiple subcellular compartments. To overcome the limitation and to further improve spatiotemporal resolutions, we have assembled a molecular toolset (CamelliA lines) in Arabidopsis ( Arabidopsis thaliana ) that enables simultaneous and high-resolution monitoring of Ca 2+ dynamics in multiple subcellular compartments through imaging different single-colored genetically encoded calcium indicators. We uncovered several Ca 2+ signatures in three types of Arabidopsis cells in response to internal and external cues, including rapid oscillations of cytosolic Ca 2+ and apical plasma membrane Ca 2+ influx in fast-growing Arabidopsis pollen tubes, the spatiotemporal relationship of Ca 2+ dynamics in four subcellular compartments of root epidermal cells challenged with salt, and a shockwave-like Ca 2+ wave propagating in laser-wounded leaf epidermis. These observations serve as a testimony to the wide applicability of the CamelliA lines for elucidating the subcellular sources contributing to the Ca 2+ signatures in plants. Abstract : A toolset for simultaneous imaging of Ca 2+ dynamics in subcellular compartments has uncovered unrecognized Ca 2+ signatures in Arabidopsis cells in response to developmental and external cues.