Multifunctionalized flower-like gold nanoparticles with high chemiluminescence for label-free sensing of the hepatitis C virus core protein. Issue 10 (14th February 2023)

• Record Type: Journal Article
• Authors: Tian, Yafei; Zhang, Yujiao; Lu, Xueyun; Xiao, Dan; Zhou, Cuisong
• Is Part Of: Journal of materials chemistry. Volume 11:Issue 10(2023)
• Page Start: 2200
• Page End: 2206
• Publication Date: 2023-02-14
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/d2tb02168d
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : The synthesis of Co 2+ /CS/Lum/AuNFs and the label-free sensing of HCVcp is reported. Abstract : Developing functionalized nanomaterials with strong chemiluminescence (CL) properties is highly significant for ultrasensitive bioanalysis. Here, we report chitosan (CS), luminol, and Co 2+ -functionalized flower-like gold nanoparticles (Co 2+ /CS/Lum/AuNFs) with strong CL for the label-free sensing of the HCV core protein (HCVcp). The Co 2+ /CS/Lum/AuNFs exhibited a greatly enhanced CL emission at around 425 nm, which is 50 times stronger than that of CS/Lum/AuNFs, and is superior to other commonly reported CL nanomaterials. The HCVcp aptamer (HCVcp-apt) further functionalized the surface of the Co 2+ /CS/Lum/AuNFs through electrostatic interactions blocked the Co 2+ catalytic site, depressing the CL. Owing to the high affinity of HCVcp for the HCVcp-apt, the presence of HCVcp predominated its binding and effectively separated the HCVcp-apt from the surface of the Co 2+ /CS/Lum/AuNFs, so that the CL intensity was significantly enhanced. As the results showed, the HCVcp-apt/Co 2+ /CS/Lum/AuNFs were successfully used to detect the HCVcp in human serum samples with a linear range from 0.50 ng mL −1 to 1.00 μg mL −1, a detection limit of 0.16 ng mL −1 and an excellent selectivity over other analogs. The strategy is universal for the development of the ultrasensitive detection of other proteins in the field of early disease diagnostics.