Genome-derived Microsatellite Markers in Lippia origanoides from Colombia. Issue 2 (3rd April 2023)

• Record Type: Journal Article
• Authors: Santamaría-Acevedo, Liliana; Levy, María Mercedes; Torres-Martínez, Lorena; Marchant, Sergio; Rondón-González, Fernando; Stashenko, Elena E.; Levy, Morris; Fuentes, Jorge Luis
• Is Part Of: Journal of herbs, spices & medicinal plants. Volume 29:Issue 2(2023)
• Page Start: 168
• Page End: 185
• Publication Date: 2023-04-03
• Subjects: Lippia origanoides -- Verbenaceae -- microsatellite -- population genetic analysis; Medicinal plants -- Periodicals; Herbs -- Periodicals; Spices -- Periodicals; 581.63
• DOI: 10.1080/10496475.2022.2121351
• Languages: English
• ISSNs: 1049-6475

Abstract

ABSTRACT: Polymorphic microsatellite sequences were mined from genome sequences of two Lippia origanoides wild specimens from disjunct locations. Twenty-three primer sequences flanking the candidate markers were designed, and their usefulness for population genetic analysis was assessed in plants sampled from four municipalities in Colombia. A final set of 10 primer pairs was useful for diversity analyses, which indicated the existence of three genetic lineages in Colombia. Cross-amplification in five related Lippia species was validated. The findings supported the efficiency of in silico approaches in providing novel genetic resources for research in Lippia species.