Multi-target Inhibitory Potency of Active Metabolites Dictates the Antimicrobial Activity of Indigenous Medicinal Plant Leucas biflora: GC-MS Analysis, Biological Evaluations, and Molecular Docking Studies. Issue 2 (3rd April 2023)

• Record Type: Journal Article
• Authors: Chatterjee, Anwesha; Acherjee, Mayukh; Das, Bijan K.; Chakraborty, Sandipan; Pal, Harshata
• Is Part Of: Journal of herbs, spices & medicinal plants. Volume 29:Issue 2(2023)
• Page Start: 134
• Page End: 144
• Publication Date: 2023-04-03
• Subjects: Indigenous medicinal plant -- disc-diffusion assay -- bioprospecting -- Leucas biflora -- molecular docking; Medicinal plants -- Periodicals; Herbs -- Periodicals; Spices -- Periodicals; 581.63
• DOI: 10.1080/10496475.2022.2116513
• Languages: English
• ISSNs: 1049-6475

Abstract

ABSTRACT: The metabolites present in the crude methanolic extract of Leucas biflora were identified and characterized to obtain new leads for compounds with antibacterial effects. The plant extract showed antibacterial effects against both the gram-positive and gram-negative bacteria in disc-diffusion assay. Gas chromatography-mass spectrometry (GC-MS) results revealed nine types of high and low molecular weight chemical entities with varying quantities in the extract. An in silico target-fishing approach identified two compounds with multi-target-directed activities. Two metabolites, 3-Oxo-18-Nor-ent-ros-4-ene-15.alpha., 16-acetonide and 4-Dehydroxy-N-(4, 5-methylenedioxy-2-nitrobenzylidene)-tyramine, inhibited two promising bacterial targets, FabH and DNA gyrase B.