Polydopamine-coated Au-Ag nanoparticle-guided photothermal colorectal cancer therapy through multiple cell death pathways. (1st January 2019)

• Record Type: Journal Article
• Authors: Hao, Miao; Kong, Chenfei; Jiang, Chengwei; Hou, Ruizhi; Zhao, Xiaoming; Li, Jing; Wang, Yuqian; Gao, Yiyao; Zhang, Hao; Yang, Bai; Jiang, Jinlan
• Is Part Of: Acta biomaterialia. Volume 83(2019)
• Page Start: 414
• Page End: 424
• Publication Date: 2019-01-01
• Subjects: NIR near-infrared -- PDA polydopamine -- NP nanoparticle -- PTT photothermal therapy -- TEM transmission electron microscopy -- CCK-8 cell counting kit -- AO acridine orange -- HSP90 heat shock protein 90 -- CsA cyclosporin A -- LMP lysosomal membrane permeability -- Pep A pepstatin A -- Leu leupeptin; Photothermal therapy -- Nanoparticles -- Lysosomal membrane permeability -- Autophagy -- Caspase-independent apoptosis; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1016/j.actbio.2018.10.032
• Languages: English
• ISSNs: 1742-7061

Abstract

Graphical abstract: Highlights: Au-Ag@PDA-mediated photothermal therapy induced apoptosis of colorectal cancer cells. Au-Ag@PDA with NIR irradiation induced autophagy in HCT-116 cells. It also induced mitochondrial damage and lysosomal membrane permeability. Photothermal exposure of Au-Ag@PDA inhibited HCT-116 tumor-bearing mouse growth. Abstract: Nanoparticles are emerging as a new therapeutic modality due to their high stability, precise targeting, and high biocompatibility. Branched Au-Ag nanoparticles with polydopamine coating (Au-Ag@PDA) have strong near-infrared absorbance and no cytotoxicity but high photothermal conversion efficiency. However, the photothermal activity of Au-Ag@PDA in vivo and in vitro has not been reported yet, and the mechanism underlying the effects of photothermal nanomaterials is not clear. Therefore, in this study, the colorectal cancer cell line HCT-116 and nude mice xenografts were used to observe the photothermal effects of Au-Ag@PDA in vivo and in vitro . The results suggest that Au-Ag@PDA NPs significantly inhibited cell proliferation and induced apoptosis in colorectal cancer cells. Moreover, Au-Ag@PDA NP-mediated photothermal therapy inhibited the growth of tumors at doses of 50 and 100 μg in vivo . The mechanisms through which Au-Ag@PDA NPs induced colorectal cancer cell death involved multiple pathways, including caspase-dependent and -independent apoptosis, mitochondrial damage, lysosomal membrane permeability, and autophagy. Thus, our findings suggest that Au-Ag@PDA NPs could be used as potential antitumor agents for photothermal ablation of colorectal cancer cells.