Functional expression of the reverse transcriptase αβ heterodimer from avian myeloblastosis virus in Escherichia coli. Issue 6 (7th April 2021)

• Record Type: Journal Article
• Authors: Makino, Yuriko; Sato, Hiroshi; Noguchi, Atsushi; Ide, Teruhiko; Yatsunami, Rie; Nakamura, Satoshi
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 85:Issue 6(2021)
• Page Start: 1464
• Page End: 1467
• Publication Date: 2021-04-07
• Subjects: avian myeloblastosis virus -- reverse transcriptase -- αβ heterodimer -- TRC reaction; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1093/bbb/zbab057
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: The α subunit of avian myeloblastosis virus reverse transcriptase (AMV-RT) is generated from the β-subunit by proteolysis, and the αβ heterodimer represents the active form. The codon-optimized gene was expressed in Escherichia coli, and an active αβ heterodimer was generated. The RNA amplification activity of the purified recombinant AMV-RT αβ heterodimer was similar to that of the native one.