A cell-engineered system to assess tumor cell sensitivity to CD8+ T cell-mediated cytotoxicity. (3rd August 2019)

• Record Type: Journal Article
• Authors: Nelson, Nadine; Lopez-Pelaez, Marta; Palazon, Asis; Poon, Edmund; De La Roche, Maike; Barry, Simon; Valge-Archer, Viia; Wilkinson, Robert W.; Dovedi, Simon J.; Smith, Paul D.
• Is Part Of: Oncoimmunology. Volume 8:Number 8(2019)
• Page Start: 1
• Page End: 10
• Publication Date: 2019-08-03
• Subjects: T cell -- cytotoxicity -- immunotherapy -- immuno-oncology; Tumors -- Immunological aspects -- Periodicals; Neoplasms -- therapy -- Periodicals; Immunotherapy -- Periodicals; 616.994
• DOI: 10.1080/2162402X.2019.1599635
• Languages: English
• ISSNs: 2162-402X

Abstract

ABSTRACT: In vitro assays that evaluate CD8 + T cell-mediated cytotoxicity are important to aid in the development of novel therapeutic approaches to enhance anti-tumor immune responses. Here, we describe a novel cytotoxicity co-culture assay that circumvents the problem of highly variable allogeneic responses and obviates the constraints of HLA-restriction between effector and target cells. We show that this assay can be easily applied to a panel of tumor cell lines to provide additional insights into intrinsic drivers of sensitivity/resistance to T cell-mediated killing, and to evaluate the impact of targeted therapies on both tumor and T cell compartments.