Isolation and plasmid characterisation of Salmonella enterica serovar Albany harbouring mcr-5 from retail chicken meat in Japan. Issue 15 (5th August 2020)

• Record Type: Journal Article
• Authors: Wakabayashi, Yuki; Sekizuka, Tsuyoshi; Yamaguchi, Takahiro; Fukuda, Akira; Suzuki, Masato; Kawahara, Ryuji; Taguchi, Masumi; Kuroda, Makoto; Semba, Keiko; Shinomiya, Hiroto; Kawatsu, Kentaro
• Is Part Of: FEMS microbiology letters. Volume 367:Issue 15(2020)
• Publication Date: 2020-08-05
• Subjects: non-typhoidal Salmonella -- colistin; mcr-5 -- whole genome sequence -- retail chicken products; Microbiology -- Periodicals; 579
• DOI: 10.1093/femsle/fnaa127
• Languages: English
• ISSNs: 0378-1097

Abstract

ABSTRACT: The emergence of plasmid-mediated colistin resistance genes ( mcr ), which is occurring in numerous countries, is a worldwide concern, primarily because colistin is a last-resort antibiotic. Compared to E. coli, prevalence of mcr genes in Salmonella is unclear in Japan. Here we screened for mcr-1–5 genes in our collection of Salmonella strains isolated from retail meat products collected in Japan from 2012 through 2016. We found that Salmonella Albany strain 27A-368 encodes mcr-5 and that mcr genes were undetectable among the remaining 202 isolates. The resistance plasmid p27A-368 was transferred by conjugation to S . Infantis and was stably retained as a transconjugant. Whole-genome sequencing revealed that mcr-5 resided on a 115 kb plasmid (p27A-368). The plasmid backbone of p27A-368 is more similar to that of pCOV27, an ESBL-encoding plasmid recovered from avian pathogenic E. coli, rather than pSE13-SA01718 of S . Paratyphi B that encodes mcr-5 . Further, mcr-5 is located on a transposon, and its sequence is similar to that of pSE13-SA01718. A phylogenetic tree based on single nucleotide variants implies a relationship between 27A-368 and S . Albany isolated in Southeast Asian countries. Abstract : A colistin-resistant Salmonella Albany strain harbouring mcr-5 on a novel IncI1-type plasmid was isolated from chicken meat in Japan.