Molecular characterization and expression patterns of CXCL8 gene from blunt snout bream (Megalobrama amblycephala) and its chemotactic effects on macrophages and neutrophils. (May 2023)

• Record Type: Journal Article
• Authors: Li, Hongping; Li, Hong; Liu, Yunlong; Zheng, Yancui; Zhang, Minying; Wang, Xu; Cui, Hujun; Wang, Haotong; Zhao, Xiaoheng; Chen, Xiangning; Cheng, Hanliang; Xu, Jianhe; Ding, Zhujin
• Is Part Of: Developmental and comparative immunology. Volume 142(2023)
• Publication Date: 2023-05
• Subjects: Megalobrama amblycephala -- CXCL8 -- Characterization -- Expression patterns -- Chemotaxis; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2023.104658
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: CXCL8 is a typical CXC-type chemokine, which mediates the migration of immune cells from blood vessels to the site of inflammation or injury to clear pathogenic microorganisms and repair damaged tissues. In this study, Megalobrama amblycephala CXCL8 ( MaCXCL8 ) gene was identified and characterized. Sequence analysis showed that the deduced MaCXCL8 protein possessed the typical structure of CXCL8 from other species, with the characteristic CXC cysteine residues in the N-terminal and accompanied by a DLR motif (Asp-Leu-Arg motif). Phylogenetic analysis revealed that MaCXCL8 was homologous to that of Ctenopharyngodon idella and other cyprinid fishes. MaCXCL8 gene was expressed in all detected healthy tissues, with the highest expression levels in the spleen, and its expression was significantly up-regulated upon the challenge of Aeromonas hydrophila and Lipopolysaccharide (LPS) both in juvenile M. amblycephala tissues and primary macrophages. The immunohistochemical assay showed that MaCXCL8 was mainly distributed in the nucleus and cytoplasm, and its expression levels increased observably with the prolongation of bacterial infection. In addition, recombinant MaCXCL8 protein exhibited significant chemotactic effects on neutrophils and macrophages. In conclusion, MaCXCL8 is involved in the immune response of M. amblycephala, and these findings will be helpful to understand the biological roles of MaCXCL8 and provide a theoretical basis for the prevention and control of fish bacterial diseases. Highlights: MaCXCL8 shared structural similarities with that of other teleosts, except DLR motif was in place of ELR-like motif. The expression of MaCXCL8 was the most abundant in the spleen, which was dramatically increased upon bacterial infection. LPS and Aeromonas hydrophila treatment significantly induced the up-regulation of MaCXCL8 expression in macrophages. Recombinant MaCXCL8 protein exhibited chemotactic effects on neutrophils and macrophages.