Gastrodin from Gastrodia elata attenuates acute myocardial infarction by suppressing autophagy: Key role of the miR-30a-5p/ATG5 pathway. (March 2023)

• Record Type: Journal Article
• Authors: Yin, Qiang; Yan, Ran; Wang, Yan; Yu, Jia
• Is Part Of: Journal of functional foods. Volume 102(2023)
• Publication Date: 2023-03
• Subjects: Autophagy -- miR-30a-5p -- Myocardial infarction -- ATG5 -- Gastrodin; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2023.105429
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Gastrodin ameliorates injuries associated with acute myocardial infarction. Gastrodin increase the level of miR-30a-5p. Gastrodin suppresses the level of ATG5. Gastrodin attenuates autophagy associated with acute myocardial infarction. Abstract: Gatrodin is an active compound with cardio-protective potentials. In the current study, the mechanism underlying the effects of gastrodin was explored. Myocardial infarction was induced and handled with gastrodin. The role of miR-30a-5p and its downstream effector, ATG5, in the cardio-protective effects of gastrodin was verified. Gastrodin induced the dys-expresseion of 72 miRs in oxygen/glucose deprivation (OGD) cardiomyocytes and miR-30a-5p was selected as the potential therapeutic target. Gastrodin increased viability and suppressed autophagy in OGD cardiomyocytes, which was associated with the induction of miR-30a-5p and deactivation of ATG5. In rat models, gastrodin attenuated structure deterioration and dysfunction in heart, and suppressed autophagy. At molecular level, miR-30a-5p was induced and ATG5 were down-regulated. After the suppression of miR-30a-5p or the overexpression of ATG5, the cardio-protective effects of gastrodin were compromised. Gastrodin induced the expression of miR-30a-5p, which contributed to the attenuation of autophagy in infarcted heart tissues.