Characterization of a novel superoxide dismutase in Nilaparvata lugens. Issue 2 (12th December 2021)

• Record Type: Journal Article
• Authors: Yamamoto, Kohji; Yamaguchi, Misuzu
• Is Part Of: Archives of insect biochemistry and physiology. Volume 109:Issue 2(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-12-12
• Subjects: antioxidant -- brown planthopper -- Nilaparvata lugens -- superoxide dismutase; Insects -- Physiology -- Periodicals; Insect biochemistry -- Periodicals; 595.701572
• DOI: 10.1002/arch.21862
• Languages: English
• ISSNs: 0739-4462

Abstract

Abstract: The brown planthopper ( Nilaparvata lugens ) is a major agricultural pest of rice crops. Analysis of the enzymes produced by N. lugens is important to develop pest‐control methods. Superoxide dismutase (SOD) is a detoxification enzyme that catalyzes the conversion of superoxide anions (reactive oxygen species) into oxygen and hydrogen peroxide. As there have been no reports on SOD in N. lugens, in this study, we characterized a new SOD in the brown planthopper, nlSOD1. Amino acid sequence and phylogenetic analyses revealed that nlSOD1 is a member of the Cu/Zn‐SOD family. Recombinant nlSOD1, when overexpressed in Escherichia coli, catalyzes the dismutation of superoxide radicals into molecular O2 and H2 O2 . Exposure to various insecticides induced nlSOD1 messenger RNA expression. These results indicate that nlSOD1 may contribute to the insecticide resistance of N. lugens . The findings of this study may assist in the development of novel methods to control the population of N. lugens . Abstract : Exposure to various insecticides induced nlSOD1 mRNA expression. We also found an increase in the amount of O2 −. nlSOD1 can reduce O2 − in response to insecticide exposure. Therefore, nlSOD1 may play a protective role against O2 − in Nilaparvata lugens . RESEARCH HIGHLIGHTS: We discovered and characterized a novel superoxide dismutase in the brown planthopper. The level of the enzyme increased upon treatment with insecticides, implying its potential role in insecticide resistance.