Full sequencing and comparison of five venom metalloproteases of Trimeresurus gracilis: The PI-enzyme is most similar to okinalysin but the PIII-enzyme is most similar to Crotalus venom enzymes. (15th March 2023)

• Record Type: Journal Article
• Authors: Tsai, Tein-Shun; Tsai, Inn-Ho
• Is Part Of: Toxicon. Volume 225(2023)
• Publication Date: 2023-03-15
• Subjects: Snake venom metalloproteases -- Disintegrins -- Sequence alignment -- Dendrogram analysis -- Venom evolution -- Trimeresurus gracilis; SVMP snake venom metalloprotease -- HPLC high performance liquid chromatography -- MALDI-TOF matrix-assisted laser desorption ionization-time of flight -- Mya million years ago -- Tgc Trimeresurus gracilis -- Ook Ovophis okinavensis; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2023.107053
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: The cDNAs encoding the Zn +2 -metalloproteases (SVMPs) of Trimeresurus gracilis (abbreviated as Tgc), a pitviper endemic to Taiwan, were cloned from venom glands and sequenced. The amino-acid sequences of five novel SVMPs, including one P-III, three P-II and one P-I class enzymes, were thus deduced and subjected to BLAST-analyses. The P-III enzyme (designated as Tgc-PIII) is structurally most similar to the PIII-SVMPs of New World pitvipers but not similar to the PIII-SVMP of Ovophis okinavensis . Sequence-similarity analysis of 22 homologous PIII-SVMPs reveal three major structural subtypes of the pitviper PIII-SVMPs, which possibly have different substrate specificities. In addition, Tgc-PIII and the PI-class SVMP (named Tgc-MP) were isolated from the venom and verified by mass spectrometry. All the three deduced sequences of PII-SVMPs (Tgc-PIIs) contain an abnormal Zn +2 -binding-site in their catalytic-domain, and an identical "long-disintegrin" domain. The predicted 85-residues disintegrin, gracilisin, bears high similarities to some long-disintegrins of the New-World pitvipers and salmosin3. By BLAST search and comparison, Tgc-MP is 96% similar to okinalysin, the hemorrhagic PI-SVMP of O. okinavensis, rather than any other PI-SVMPs in the databanks. Our results confirm the fast evolution of Tgc-SVMPs as well as their structural similarities to different SVMP-classes of the New-World pitvipers and of O. okinavensis, respectively. The implications of our findings are discussed along with our previous sequence comparisons of venom phospholipases A2 and ten venom serine proteases of Tgc. Graphical abstract: Image 1 Highlights: Five T. gracilis metalloproteases, including PI, PII & PIII, were fully sequenced. Both the PI & PIII were purified from venom and verified by mass spectrometry. The PI sequence is most similar to that of O. okinavensis venom PI-enzyme. The PIII sequence is most similar to those of New World pitvipers. All three PII-variants contain an identical long-disintegrin domain of 85 residues.