A seminal perspective on the role of chondroitin sulfate in biomineralization. (15th June 2023)

• Record Type: Journal Article
• Authors: Hao, Jia-xin; Wan, Qian-qian; Mu, Zhao; Gu, Jun-ting; Yu, Wei-wei; Qin, Wen; Li, Yu-tao; Wang, Chen-yu; Ma, Yu-xuan; Jiao, Kai; Tay, Franklin; Niu, Lina
• Is Part Of: Carbohydrate polymers. Volume 310(2023)
• Publication Date: 2023-06-15
• Subjects: ACP amorphous calcium phosphate -- CaP calcium phosphate -- ChABC chondroitinase ABC -- CLSM confocal laser scanning microscopy -- CS chondroitin sulfate -- CS-ACP CS-stabilized amorphous calcium phosphate -- DLS dynamic light scattering -- ECM extracellular matrix -- EDC 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride -- EDX energy dispersive X-ray -- FTIR Fourier-transform infrared spectroscopy -- GAG glycosaminoglycans -- HAADF-STEM high-angle annular dark-field scanning transmission electron microscopy -- HO heterotopic ossification -- HPLC high performance liquid chromatography -- HYAL1 hyaluronidase-1 -- MD molecular dynamics -- NHS N-hydroxysulfosuccinimide -- Rg radius of gyration -- SAED selected area electron diffraction -- SEM scanning electron microscopy -- TEM transmission electron microscopy; Amorphous calcium phosphate -- Chondroitin sulfate -- Heterotopic ossification -- Intrafibrillar mineralization -- Proteoglycans; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2023.120738
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Chondroitin sulfate (CS) is an important extracellular matrix component of mineralized tissues. It participates in biomineralization, osteoblast differentiation and promotes bone tissue repair in vitro . However, the mechanism in which CS functions is unclear. Accordingly, an in-depth investigation of how CS participates in mineralization was conducted in the present study. Chondroitin sulfate was found to directly induce intrafibrillar mineralization of the collagen matrix. The mineralization outcome was dependent on whether CS remained free in the extracellular matrix or bound to core proteins; mineralization only occurred when CS existed in a free state. The efficacy of mineralization appeared to increase with ascending CS concentration. This discovery spurred the authors to identify the cause of heterotopic ossification in the Achilles tendon. Chondroitin sulfate appeared to be a therapeutic target for the management of diseases associated with heterotopic calcification. A broader perspective was presented on the applications of CS in tissue engineering. Graphical abstract: Unlabelled Image