Decrease in hemicellulose content and its retention of iron contributes to phosphorus deficiency alleviated iron deficiency in Arabidopsis thaliana. (April 2023)

• Record Type: Journal Article
• Authors: Li, Su; Zhang, Yue; Wu, Qi; Huang, Jing; Shen, Ren Fang; Zhu, Xiao Fang
• Is Part Of: Plant science. Volume 329(2023)
• Publication Date: 2023-04
• Subjects: Fe deficiency -- P deficiency -- Cell wall -- Hemicellulose 1 -- Reutilization; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2023.111605
• Languages: English
• ISSNs: 0168-9452

Abstract

Abstract: The physiological and molecular mechanisms between phosphorus (P) and iron (Fe) interactions are still elusive although they have been extensively investigated. In this study, we uncovered that limiting P supply could alleviate Fe deficiency in Arabidopsis (Col-0). Under Fe deficiency, P deficiency (-Fe-P) decreased cell wall Fe accumulation in root, but elevated Fe accumulation in the shoot, implying that the reduced Fe retention in the root cell wall may contribute to the P-deficiency-alleviated Fe deficiency in the shoot. On the other hand, increasing P supply could mimic the degree of Fe deficiency in terms of the expressions of genes induced after Fe deficient treatment. The components of the root cell wall showed that there was no distinction in the pectin content and the Fe retention in pectin between -Fe and -Fe-P treatments, while hemicellulose 1 content and Fe retained in it were decreased significantly in -Fe-P treatment as compared with -Fe treatment. The time-course experiment showed that decreasing cell wall retained Fe was mainly from the corresponding decrease in hemicellulose 1 retained Fe. Furthermore, the up-regulation of IRT1 expression in -Fe-P was obviously lower than -Fe. All these suggest that the P deficiency-induced decrease of hemicellulose 1 component leads to reutilization of root cell wall Fe and improvement of Fe nutrition in shoot in Fe deficient Arabidopsis. Our results provide a novel explanation of the interplay between Fe and P in Arabidopsis. Highlight: ● P deficiency alleviates Fe deficiency in Arabidopsis thaliana . ● P status affects the expression of Fe uptake related genes. ● P deficiency stimulates Fe release from the root cell wall. ● P deficiency decreases hemicellulose 1 content in the root cell wall. ● Potential correlation of the hemicellulose Fe content and Fe-homeostasis.