A Carbon Paste Electrode Modified by Bentonite and l‐Cysteine for Simultaneous Determination of Ascorbic and Uric Acids: Application in Biological Fluids. Issue 2 (1st February 2023)

• Record Type: Journal Article
• Authors: Choukairi, Mohamed; Bouchta, Dounia; Bounab, Loubna; González‐Romero, Elisa; Achache, Mohamed; Draoui, Khalid; Chaouket, Faiza; Raissouni, Ihssane; Gharous, Mouad
• Is Part Of: ChemistryOpen. Volume 12:Issue 2(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-02-01
• Subjects: ascorbic acid -- bentonite -- carbon paste electrode -- l-cysteine -- uric acid; Chemistry -- Periodicals; 540; 540.5
• DOI: 10.1002/open.202200201
• Languages: English
• ISSNs: 2191-1363

Abstract

Abstract: A novel modification of a paste carbon electrode by Bentonite (Bent) and l ‐Cysteine (l ‐Cyst) was carried out for uric acid (UA) and ascorbic acid (AA) detection and quantification. Morphological and compositional characterization of the electrode surface were carried out using electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and energy dispersive X‐ray spectroscopic analysis (EDS). Cyclic voltammetry (CV) and square wave voltammetry (SWV) techniques were used to analyze UA and AA. The obtained sensor shows a good stability, sensibility, selectivity, and regeneration ability. Accordingly, the limit of detection (LOD) is found to be 0.031 μm and 9.6 μm for UA and AA, respectively. A good linearity in the range of 0.1 to 100 μm for UA and 10 to 1000 μm for AA was obtained. The peak‐to‐peak separation of UA‐AA (Δ E UA‐AA ) was determined to be 330 mV. In addition, the sensor is applied successfully to monitor UA and AA in serum samples. Abstract : A l ‐Cyst/Bent/CPE modified electrode was prepared for uric acid (UA) and ascorbic acid (AA) analysis in chemical and biological environments. The modified sensor showed a good stability, selectivity, regeneration ability, very remarkable reproducibility, and competitive limits of detection and quantification. In addition, the sensor was successfully applied to monitor UA and AA individually and simultaneously, and can be effectively used for the detection of UA and AA in real samples.