Metabolic profiling of colorectal cancer organoids: A comparison between high‐resolution magic angle spinning magnetic resonance spectroscopy and solution nuclear magnetic resonance spectroscopy of polar extracts. (22nd December 2022)

• Record Type: Journal Article
• Authors: van der Kemp, Wybe J. M.; Grinde, Maria T.; Malvik, Jon O.; van Laarhoven, Hanneke W. M.; Prompers, Jeanine J.; Klomp, Dennis W. J.; Burgering, Boudewijn; Bathen, Tone Frost; Moestue, Siver Andreas
• Other Names: Bathen Tone F. guestEditor.; Cheng Leo L. guestEditor.
• Is Part Of: NMR in biomedicine. Volume 36:Number 4(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-12-22
• Subjects: colorectal cancer, HR MAS MRS, lactate, myo‐inositol, NMR, organoids, phosphocholine, tumor progression organoids; Nuclear magnetic resonance -- Periodicals; Magnetic Resonance Spectroscopy -- Periodicals; 574
• DOI: 10.1002/nbm.4882
• Languages: English
• ISSNs: 0952-3480

Abstract

Abstract: Patient‐derived cancer cells cultured in vitro are a cornerstone of cancer metabolism research. More recently, the introduction of organoids has provided the research community with a more versatile model system. Physiological structure and organization of the cell source tissue are maintained in organoids, representing a closer link to in vivo tumor models. High‐resolution magic angle spinning magnetic resonance spectroscopy (HR MAS MRS) is a commonly applied analytical approach for metabolic profiling of intact tissue, but its use has not been reported for organoids. The aim of the current work was to compare the performance of HR MAS MRS and extraction‐based nuclear magnetic resonance (NMR) in metabolic profiling of wild‐type and tumor progression organoids (TPOs) from human colon cancer, and further to investigate how the sequentially increased genetic alterations of the TPOs affect the metabolic profile. Sixteen metabolites were reliably identified and quantified both in spectra based on NMR of extracts and HR MAS MRS of intact organoids. The metabolite concentrations from the two approaches were highly correlated (r = 0.94), and both approaches were able to capture the systematic changes in metabolic features introduced by the genetic alterations characteristic of colorectal cancer progression (e.g., increased levels of lactate and decreased levels of myo‐inositol and phosphocholine with an increasing number of mutations). The current work highlights that HR MAS MRS is a well‐suited method for metabolic profiling of intact organoids, with the additional benefit that the nondestructive nature of HR MAS enables subsequent recovery of the organoids for further analyses based on nucleic acids or proteins. Abstract : Tumor progression organoids (TPOs) representing the genetic alterations in colorectal cancer were analyzed by two methods: extraction NMR and high‐resolution magic angle spinning (HR MAS) MRS. Correlation analysis and principal component analysis from MR spectra show that the two methods can be useful in the metabolic profiling of organoids. NMR analysis of the TPOs indicates that metabolic changes occur at an early stage during tumor progression.