Proteomic profiling of epicardial fat in heart failure with preserved versus reduced and mildly reduced ejection fraction. Issue 5 (20th February 2023)

• Record Type: Journal Article
• Authors: Gao, Qian; He, Shan; Peng, Yuanshu; Su, Pixiong; Zhao, Lei
• Is Part Of: Journal of cellular and molecular medicine. Volume 27:Issue 5(2023)
• Page Start: 727
• Page End: 735
• Publication Date: 2023-02-20
• Subjects: epicardial adipose tissue -- heart failure with mildly reduced ejection fraction -- heart failure with preserved ejection fraction -- heart failure with reduced ejection fraction -- proteomics; Cytology; Medicine; Molecular Biology; Cytologie -- Périodiques; Médecine -- Périodiques; Biologie moléculaire -- Périodiques; Cytology -- Periodicals; Medicine -- Periodicals; Molecular biology -- Periodicals; 611.01805
• DOI: 10.1111/jcmm.17695
• Languages: English
• ISSNs: 1582-1838

Abstract

Abstract: In order to explore the proteomic signatures of epicardial adipose tissue (EAT) related to the mechanism of heart failure with reduced and mildly reduced ejection fraction (HFrEF/HFmrEF) and heart failure (HF) with preserved ejection fraction (HFpEF), a comprehensive proteomic analysis of EAT was made in HFrEF/HFmrEF ( n = 5) and HFpEF ( n = 5) patients with liquid chromatography–tandem mass spectrometry experiments. The selected differential proteins were verified between HFrEF/HFmrEF ( n = 20) and HFpEF ( n = 40) by ELISA (enzyme‐linked immunosorbent assay). A total of 599 EAT proteins were significantly different in expression between HFrEF/HFmrEF and HFpEF. Among the 599 proteins, 58 proteins increased in HFrEF/HFmrEF compared to HFpEF, whereas 541 proteins decreased in HFrEF/HFmrEF. Of these proteins, TGM2 in EAT was down‐regulated in HFrEF/HFmrEF patients and was confirmed to decrease in circulating plasma of the HFrEF/HFmrEF group ( p = 0.019). Multivariate logistic regression analysis confirmed plasma TGM2 could be an independent predictor of HFrEF/HFmrEF ( p = 0.033). Receiver operating curve analysis indicated that the combination of TGM2 and Gensini score improved the diagnostic value of HFrEF/HFmrEF ( p = 0.002). In summary, for the first time, we described the proteome in EAT in both HFpEF and HFrEF/HFmrEF and identified a comprehensive dimension of potential targets for the mechanism behind the EF spectrum. Exploring the role of EAT may offer potential targets for preventive intervention of HF.