High‐resolution magic angle spinning NMR for intact biological specimen analysis: Initial discovery, recent developments, and future directions. (8th February 2022)

• Record Type: Journal Article
• Authors: Cheng, Leo L.
• Other Names: Bathen Tone F. guestEditor.; Cheng Leo L. guestEditor.
• Is Part Of: NMR in biomedicine. Volume 36:Number 4(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-02-08
• Subjects: cells -- high‐resolution magic angle spinning -- intact tissue -- metabolomics; Nuclear magnetic resonance -- Periodicals; Magnetic Resonance Spectroscopy -- Periodicals; 574
• DOI: 10.1002/nbm.4684
• Languages: English
• ISSNs: 0952-3480

Abstract

Abstract : High‐resolution magic angle spinning (HRMAS) NMR, an approach for intact biological material analysis discovered more than 25 years ago, has been advanced by many technical developments and applied to many biomedical uses. This article provides a history of its discovery, first by explaining the key scientific advances that paved the way for HRMAS NMR's invention, and then by turning to recent developments that have profited from applying and advancing the technique during the last 5 years. Developments aimed at directly impacting healthcare include HRMAS NMR metabolomics applications within studies of human disease states such as cancers, brain diseases, metabolic diseases, transplantation medicine, and adiposity. Here, the discussion describes recent HRMAS NMR metabolomics studies of breast cancer and prostate cancer, as well as of matching tissues with biofluids, multimodality studies, and mechanistic investigations, all conducted to better understand disease metabolic characteristics for diagnosis, opportune windows for treatment, and prognostication. In addition, HRMAS NMR metabolomics studies of plants, foods, and cell structures, along with longitudinal cell studies, are reviewed and discussed. Finally, inspired by the technique's history of discoveries and recent successes, future biomedical arenas that stand to benefit from HRMAS NMR‐initiated scientific investigations are presented. Abstract : High‐resolution magic angle spinning (HRMAS) NMR, an approach for intact biological material analysis that was discovered more than 25 years ago (in 1996), has been advanced by many technical developments and applied to many biomedical uses. The typical size of intact tissue samples and NMR rotor and inserts used in the HRMAS NMR study (Cheng LL. eMagRes . 2013;2:79‐88. DOI 10.1002/9780470034590.emrstm1231).