Real‐Time Dissection of the Transportation and miRNA‐Release Dynamics of Small Extracellular Vesicles. Issue 7 (4th January 2023)

• Record Type: Journal Article
• Authors: Xia, Hou‐Fu; Yu, Zi‐Li; Zhang, Li‐Juan; Liu, Shu‐Lin; Zhao, Yi; Huang, Jue; Fu, Dan‐Dan; Xie, Qi‐Hui; Liu, Hai‐Ming; Zhang, Zhi‐Ling; Zhao, Yi‐Fang; Wu, Min; Zhang, Wei; Pang, Dai‐Wen; Chen, Gang
• Is Part Of: Advanced science. Volume 10:Issue 7(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-01-04
• Subjects: angiogenesis -- extracellular vesicles -- miRNA -- quantum dots -- single particle tracking; Science -- Periodicals; 505
• DOI: 10.1002/advs.202205566
• Languages: English
• ISSNs: 2198-3844

Abstract

Abstract: Extracellular vesicles (EVs) are cell‐derived membrane‐enclosed structures that deliver biomolecules for intercellular communication. Developing visualization methods to elucidate the spatiotemporal dynamics of EVs' behaviors will facilitate their understanding and translation. With a quantum dot (QD) labeling strategy, a single particle tracking (SPT) platform is proposed here for dissecting the dynamic behaviors of EVs. The interplays between tumor cell‐derived small EVs (T‐sEVs) and endothelial cells (ECs) are specifically investigated based on this platform. It is revealed that, following a clathrin‐mediated endocytosis by ECs, T‐sEVs are transported to the perinuclear region in a typical three‐stage pattern. Importantly, T‐sEVs frequently interact with and finally enter lysosomes, followed by quick release of their carried miRNAs. This study, for the first time, reports the entire process and detailed dynamics of T‐sEV transportation and cargo‐release in ECs, leading to better understanding of their proangiogenic functions. Additionally, the QD‐based SPT technique will help uncover more secrets of sEV‐mediated cell–cell communication. Abstract : By developing the quantum dot‐based multicomponent labeling strategy and assembling the single particle tracking platform, for the first time, the three‐stage transportation process is tracked following the clathrin‐mediated endocytosis of tumor‐derived small extracellular vesicles (sEVs) in endothelial cells and their miRNA cargo‐release details are recorded further in real time.