Premna integrifolia Linn: Phytochemical Profile and Evaluation of Antiproliferative Effects. Issue 9 (28th February 2023)

• Record Type: Journal Article
• Authors: Rahman, Atiqur; Siddiqui, Shah Alam; Altun, Muhammed; Demirtas, Ibrahim
• Is Part Of: ChemistrySelect. Volume 8:Issue 9(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-02-28
• Subjects: apoptosis -- cancer -- natural products -- phytochemical characterization -- total phenolics; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.202204854
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: Premna integrifolia (PI) is a popular ethnomedicinal plant in Indian subcontinent and used for treating different types of ailments. Here, organic leaf extracts of PI have been studied for exploring phytochemical compositions and anti‐proliferative properties of those extracts. The extracts have been investigated qualitatively and quantitatively by HPLC/TOF‐MS, and fourteen phenolic compounds were identified including eupatorin, 4‐hydroxybenzoic acid, diosmin, and apigenin as major compounds. The GC/MS study of hexane extract identified 9‐octadecenoic acid, palmitic acid, phytol and stearic acid as main volatile organic compounds. xCELLigence (Real Time Cell Analyzer) has been used to study effects of different extracts on human cervical cancer cell lines (HeLa) at various concentrations. The chloroform and ethyl acetate extracts exhibited strong antiproliferative activities as a dose dependent manner. Our study explored PI as a source of biochemically active compounds having therapeutic potential as antiproliferative agent against cancer cells. Abstract : Phytochemical composition and anticancer potential of Premna Integrifolia : Premna Integrifolia is a popular ethnomedicinal plant in Indian subcontinent. Effective use of the plants in treating different diseases has necessitate exploring its phytochemicals and scientifically studying biological efficacy of the plant. Our study using HPLC/TOF‐MS and GC‐MS revealed the plant as a rich source of different biologically active phytochemicals e. g. polyphenols, volatile compounds and fatty acids. The plant also found to exhibit very promising effect against cancer cells in anticancer assessment using xCELLigence (Real Time Cell Analyzer