Potential plasma microRNAs signature miR-190b-5p, miR-215-5p and miR-527 as non-invasive biomarkers for prostate cancer. (17th February 2023)

• Record Type: Journal Article
• Authors: Khan, Mohd Mabood; Serajuddin, Mohammad; Bharadwaj, Mausumi
• Is Part Of: Biomarkers. Volume 28:Number 2(2023)
• Page Start: 227
• Page End: 237
• Publication Date: 2023-02-17
• Subjects: Prostate cancer -- microRNA -- differentially expressed miRNA -- plasma -- ROC; Biochemical markers -- Periodicals; 610.28
• DOI: 10.1080/1354750X.2022.2163694
• Languages: English
• ISSNs: 1354-750X

Abstract

Abstract: Background Prostate cancer (PCa) is the most prevalent (20%) pathological cancer among males globally. MicroRNAs (miRNAs) are short (19–22 nucleotide), conserved, noncoding molecules that regulate post-transcriptional processes either by repressing or degrading mRNA or by translation inhibition binding to complementary sites on mRNA. The goal of this study was to find out whether differentially expressed microRNA (DEM) could be used as a potential marker in the prognosis and diagnosis of PCa. Methodology The miRNAs profiling was done both from plasma and tissue samples of the same PCa patient (n = 3) by real-time quantitative PCR (qRT-PCR) and compared with BPH (benign prostatic hyperplasia) patients (n = 3) as controls and further validation of selected miRNAs. Results We found 55 significant overexpressed DEMs, 44 significant underexpressed DEMs in plasma and 6 significant overexpressed DEMs, 27 significant underexpressed DEMs in tissue compared between PCa and BPH. Furthermore, there were eight miRNAs namely miR-190b, miR-215, miR-300, miR-329, miR-504, miR-525-3p, miR-527, miR-548a-3p found to be significantly differentially expressed in plasma and tissue samples via profiling, however only three showed concordant expression. After validation, miR-190b-5p were shown to be significantly downexpressed with fold changes of 0.4177 (p value – 0.0072) and 0.7264 (p value – 0.0143) in plasma and tissue samples, respectively. The expression of miR-215-5p was shown to be significantly overexpressed with fold change of 1.820 (p – 0.0016) and 1.476 (p – 0.0407) in plasma and tissue samples, respectively. Furthermore, miR-527 was shown to be significantly downexpressed with fold changes of 0.6018 (p – 0.0095) and 0.6917 (p – 0.0155) in plasma and tissue samples, respectively. Conclusion According to our findings, plasma miR-190b-5p, miR-215-5p, miR-527 levels alteration is consistently linked with PCa tissue. For establishing significant miRNAs as biomarkers, additional research of a larger population is needed.