Conformational-switch biosensors as novel tools to support continuous, real-time molecular monitoring in lab-on-a-chip devices. Issue 5 (19th January 2023)

• Record Type: Journal Article
• Authors: Parolo, Claudio; Idili, Andrea; Heikenfeld, Jason; Plaxco, Kevin W.
• Is Part Of: Lab on a chip. Volume 23:Issue 5(2023)
• Page Start: 1339
• Page End: 1348
• Publication Date: 2023-01-19
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/d2lc00716a
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : We argue that the introduction of high-frequency, real-time biosensors into LoCs could be used to establish high-value feed-back control functionalities. Abstract : Recent years have seen continued expansion of the functionality of lab on a chip (LOC) devices. Indeed LOCs now provide scientists and developers with useful and versatile platforms across a myriad of chemical and biological applications. The field still fails, however, to integrate an often important element of bench-top analytics: real-time molecular measurements that can be used to "guide" a chemical response. Here we describe the analytical techniques that could provide LOCs with such real-time molecular monitoring capabilities. It appears to us that, among the approaches that are general ( i.e., that are independent of the reactive or optical properties of their targets), sensing strategies relying on binding–induced conformational change of bioreceptors are most likely to succeed in such applications.