Quantitative analysis of fucosylated glycoproteins by immobilized lectin-affinity fluorescent labeling. Issue 10 (27th February 2023)

• Record Type: Journal Article
• Authors: Gao, Ziyuan; Chen, Sufeng; Du, Jing; Wu, Zhen; Ge, Wei; Gao, Song; Zhou, Zeyang; Yang, Xiaodong; Xing, Yufei; Shi, Minhua; Hu, Yunyun; Tang, Wen; Xia, Jun; Zhang, Xumin; Jiang, Junhong; Yang, Shuang
• Is Part Of: RSC advances. Volume 13:Issue 10(2023)
• Page Start: 6676
• Page End: 6687
• Publication Date: 2023-02-27
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d3ra00072a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : The LAFLQ method quantifies glycoproteins by fluorophore labeling and lectin affinity. On-plate fluorescence detection enables simultaneous analysis of multiple samples. Glycosylations in human biofluids can be achieved using different lectins. Abstract : Human biofluids are often used to discover disease-specific glycosylation, since abnormal changes in protein glycosylation can discern physiopathological states. Highly glycosylated proteins in biofluids make it possible to identify disease signatures. Glycoproteomic studies on saliva glycoproteins showed that fucosylation was significantly increased during tumorigenesis and that glycoproteins became hyperfucosylated in lung metastases, and tumor stage is associated with fucosylation. Quantification of salivary fucosylation can be achieved by mass spectrometric analysis of fucosylated glycoproteins or fucosylated glycans; however, the use of mass spectrometry is non-trivial for clinical practice. Here, we developed a high-throughput quantitative method, lectin-affinity fluorescent labeling quantification (LAFLQ), to quantify fucosylated glycoproteins without relying on mass spectrometry. Lectins with a specific affinity for fucoses are immobilized on the resin and effectively capture fluorescently labeled fucosylated glycoproteins, which are further quantitatively characterized by fluorescence detection in a 96-well plate. Our results demonstrated that serum IgG can be accurately quantified by lectin and fluorescence detection. Quantification in saliva showed significantly higher fucosylation in lung cancer patients compared to healthy controls or other non-cancer diseases, suggesting that this method has the potential to quantify stage-related fucosylation in lung cancer saliva.