Critical role for thymic CD19+CD5+CD1dhiIL-10+ regulatory B cells in immune homeostasis. Issue 3 (16th December 2014)

• Record Type: Journal Article
• Authors: Xing, Chen; Ma, Ning; Xiao, He; Wang, Xiaoqian; Zheng, Mingke; Han, Gencheng; Chen, Guojiang; Hou, Chunmei; Shen, Beifen; Li, Yan; Wang, Renxi
• Is Part Of: Journal of leukocyte biology. Volume 97:Issue 3(2015)
• Page Start: 547
• Page End: 556
• Publication Date: 2014-12-16
• Subjects: regulatory T cells -- Foxp3 -- CD72; Leucocytes -- Periodicals; Reticulo-endothelial system -- Periodicals; 571.96
• DOI: 10.1189/jlb.3A0414-213RR
• Languages: English
• ISSNs: 0741-5400

Abstract

Abstract : Thymic CD19 + CD5 + CD1d hi IL-10 + B cells play a critical role in the maintenance of immune homeostasis. Abstract: This study tested the hypothesis that besides the spleen, LNs, peripheral blood, and thymus contain a regulatory IL-10-producing CD19 + CD5 + CD1d high B cell subset that may play a critical role in the maintenance of immune homeostasis. Indeed, this population was identified in the murine thymus, and furthermore, when cocultured with CD4 + T cells, this population of B cells supported the maintenance of CD4 + Foxp3 + Tregs in vitro, in part, via the CD5–CD72 interaction. Mice homozygous for Cd19 Cre (CD19 −/− ) express B cells with impaired signaling and humoral responses. Strikingly, CD19 −/− mice produce fewer CD4 + Foxp3 + Tregs and a greater percentage of CD4 + CD8 − and CD4 − CD8 + T cells. Consistent with these results, transfer of thymic CD19 + CD5 + CD1d hi B cells into CD19 −/− mice resulted in significantly up-regulated numbers of CD4 + Foxp3 + Tregs with a concomitant reduction in CD4 + CD8 − and CD4 − CD8 + T cell populations in the thymus, spleen, and LNs but not in the BM of recipient mice. In addition, thymic CD19 + CD5 + CD1d hi B cells significantly suppressed autoimmune responses in lupus-like mice via up-regulation of CD4 + Foxp3 + Tregs and IL-10-producing Bregs . This study suggests that thymic CD19 + CD5 + CD1d hi IL-10 + Bregs play a critical role in the maintenance of immune homeostasis.