Human mast cells capture, store, and release bioactive, exogenous IL-17A. Issue 3 (31st March 2016)

• Record Type: Journal Article
• Authors: Noordenbos, Troy; Blijdorp, Iris; Chen, Sijia; Stap, Jan; Mul, Erik; Cañete, Juan D; Lubberts, Erik; Yeremenko, Nataliya; Baeten, Dominique
• Is Part Of: Journal of leukocyte biology. Volume 100:Issue 3(2016)
• Page Start: 453
• Page End: 462
• Publication Date: 2016-03-31
• Subjects: spondyloarthritis -- mRNA expression -- uptake; Leucocytes -- Periodicals; Reticulo-endothelial system -- Periodicals; 571.96
• DOI: 10.1189/jlb.3HI1215-542R
• Languages: English
• ISSNs: 0741-5400

Abstract

Abstract : Mast cells cannot produce, but take up exogenous IL-17A. Abstract: IL-17A, a major proinflammatory cytokine, can be produced by a variety of leukocytes, but its exact cellular source in human inflammatory diseases remains incompletely understood. IL-17A protein is abundantly found in mast cells in human tissues, such as inflamed synovium, but surprisingly, mechanistic murine studies failed to demonstrate IL-17A production by mast cells. Here, we demonstrate that primary human tissue mast cells do not produce IL-17A themselves but actively capture exogenous IL-17A through receptor-mediated endocytosis. The exogenous IL-17A is stored in intracellular granules and can subsequently be released in a bioactive form. This novel mechanism confers to mast cells the capacity to steer IL-17A-mediated tissue inflammation by the rapid release of preformed cytokine.