Frontline Science: A hyporesponsive subset of rat NK cells negative for Ly49s3 and NKR-P1B are precursors to the functionally mature NKR-P1B+ subset. Issue 6 (26th July 2017) - Sudworth, Amanda
Abstract : Functional competence of hyporesponsive rat NK cells is associated with acquisition of the inhibitory receptor NKR-P1B. Abstract: Rat NK cells are divided into major subsets expressing either Ly49 receptors or the inhibitory NKR-P1B receptor in conjunction with NKG2A/C/E receptors. A minor subset of NKp46 + cells lacking expression of both Ly49 receptors and NKR-P1B is present in blood and spleen and is associated with decreased functional competence. We hypothesized that this subset may represent precursors to Ly49 + and/or NKR-P1B + NK cells. When cultured in vitro in IL-2 and IL-15 or adoptively transferred to syngeneic hosts, a portion of NKR-P1B − Ly49s3 − cells transformed to express NKR-P1B, but very little Ly49s3. Acquisition of NKR-P1B by NKR-P1B − Ly49s3 − cells coincided with increased degranulation. In addition, although NKR-P1B − Ly49s3 − cells highly proliferate, proliferative activity was reduced upon acquisition of NKR-P1B at comparable levels to bona fide NKR-P1B + NK cells. A fraction of NKR-P1B − Ly49s3 − cells remained negative for NKR-P1B, both in vitro and after adoptive transfer in vivo. Most NKR-P1B − Ly49s3 − cells expressed the transcription factor Eomesodermin and NK cell markers, indicating that these cells represent conventional NK cells. Our findings suggest that the NKR-P1B − Ly49s3 − NK cells are precursors to NKR-P1B single-positive cells and that functional competence is acquired upon expression of NKR-P1B.